Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
25469-1-AP targets PNPLA4 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PNPLA4 fusion protein Ag22091 相同性解析による交差性が予測される生物種 |
| Full Name | patatin-like phospholipase domain containing 4 |
| Calculated molecular weight | 253 aa, 28 kDa |
| Observed molecular weight | 28 kDa |
| GenBank accession number | BC020746 |
| Gene Symbol | PNPLA4 |
| Gene ID (NCBI) | 8228 |
| RRID | AB_2880095 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P41247 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PNPLA4(Patatin-like phospholipase domain-containing protein 4) is also named as DXS1283E, GS2. It participate in triacylglycerol hydrolysis and the acyl-CoA independent transacylation of acylglycerols, there by facilitating energy mobilization and storage in adipocytes. This protein has 2 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PNPLA4 antibody 25469-1-AP | Download protocol |
| IHC protocol for PNPLA4 antibody 25469-1-AP | Download protocol |
| IP protocol for PNPLA4 antibody 25469-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Lysophosphatidylserine induces necrosis in pressure overloaded male mouse hearts via G protein coupled receptor 34 |