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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PNO1 Recombinant antibody (85834-4-RR)

    WB analysis using 85834-4-RR (same clone as 85834-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85834-4-RR (PNO1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85834-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using PNO1 Recombinant antibody (85834-4-RR)

    IF Staining of A549 using 85834-4-RR (same clone as 85834-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using PNO1 antibody (85834-4-RR, Clone: 250134F6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85834-4-PBS in a different storage buffer formulation.

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PNO1 Recombinant antibody, PBS Only

PNO1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 85834-4-PBS
Clone No.250134F6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

KHRBP1, Partner of NOB1, RNA binding protein PNO1, RNA-binding protein PNO1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1 mg/ml
100 μg, 1 mg/ml

¥167,000/ 100 μg

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85834-4-PBS targets PNO1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PNO1 fusion protein Ag14752 相同性解析による交差性が予測される生物種
Full Name partner of NOB1 homolog (S. cerevisiae)
Calculated molecular weight 252 aa, 28 kDa
Observed molecular weight 28-35 kDa
GenBank accession numberBC008304
Gene Symbol PNO1
Gene ID (NCBI) 56902
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NRX1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Human RNA binding gene partner of NOB1 (PNO1) was firstly isolated from human kidney and encodes 35-kDa protein, named PNO1. PNO1 as a ribosome biogenesis factor that contributes to 40S subunit assembly by binding with its partner NOB1. PNO1 is a highly conserved protein with a K homology (KH) domain at its C-terminal and two putative nuclear localization signals at its N-terminal . PNO1 also had great regulatory function in tumor metabolism. (PMID: 38071381, PMID: 33864661)