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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using PNMA1 Monoclonal antibody (68415-1-Ig)

    WB analysis of PC-3 using 68415-1-Ig (same clone as 68415-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 68415-1-Ig (PNMA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68415-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of LNCaP cells using PNMA1 Monoclonal antibody (68415-1-Ig)

    WB analysis of LNCaP using 68415-1-Ig (same clone as 68415-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68415-1-Ig (PNMA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68415-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat testis tissue using PNMA1 Monoclonal antibody (68415-1-Ig)

    WB analysis of rat testis using 68415-1-Ig (same clone as 68415-1-PBS)

    rat testis tissue were subjected to SDS PAGE followed by western blot with 68415-1-Ig (PNMA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68415-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of T-47D cells using PNMA1 Monoclonal antibody (68415-1-Ig)

    WB analysis of T-47D using 68415-1-Ig (same clone as 68415-1-PBS)

    T-47D cells were subjected to SDS PAGE followed by western blot with 68415-1-Ig (PNMA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68415-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using PNMA1 Monoclonal antibody (68415-1-Ig)

    IF Staining of PC-3 using 68415-1-Ig (same clone as 68415-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using PNMA1 antibody (68415-1-Ig, Clone: 2D8C6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68415-1-PBS in a different storage buffer formulation.

PNMA1 Monoclonal antibody, PBS Only

PNMA1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68415-1-PBS
Clone No.2D8C6

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Paraneoplastic antigen Ma1, MA1, 37 kDa neuronal protein, 2D8C6

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68415-1-PBS targets PNMA1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag33143

Product name: Recombinant human PNMA1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-353 aa of BC039577

Sequence: MAMTLLEDWCRGMDVNSQRALLVWGIPVNCDEAEIEETLQAAMPQVSYRMLGRMFWREENAKAALLELTGAVDYAAIPREMPGKGGVWKVLFKPPTSDAEFLERLHLFLAREGWTVQDVARVLGFQNPTPTPGPEMPAEMLNYILDNVIQPLVESIWYKRLTLFSGRDIPGPGEETFDPWLEHTNEVLEEWQVSDVEKRRRLMESLRGPAADVIRILKSNNPAITTAECLKALEQVFGSVESSRDAQIKFLNTYQNPGEKLSAYVIRLEPLLQKVVEKGAIDKDNVNQARLEQVIAGANHSGAIRRQLWLTGAGEGPAPNLFQLLVQIREEEAKEEEEEAEATLLQLGLEGHF

相同性解析による交差性が予測される生物種
Full Name paraneoplastic antigen MA1
Calculated molecular weight 353 aa, 40 kDa
Observed molecular weight37 kDa
GenBank accession numberBC039577
Gene Symbol PNMA1
Gene ID (NCBI) 9240
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ8ND90
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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