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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using PNMA1 Monoclonal antibody (68415-1-Ig)

    WB analysis of PC-3 using 68415-1-Ig (same clone as 68415-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 68415-1-Ig (PNMA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68415-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of LNCaP cells using PNMA1 Monoclonal antibody (68415-1-Ig)

    WB analysis of LNCaP using 68415-1-Ig (same clone as 68415-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68415-1-Ig (PNMA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68415-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat testis tissue using PNMA1 Monoclonal antibody (68415-1-Ig)

    WB analysis of rat testis using 68415-1-Ig (same clone as 68415-1-PBS)

    rat testis tissue were subjected to SDS PAGE followed by western blot with 68415-1-Ig (PNMA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68415-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of T-47D cells using PNMA1 Monoclonal antibody (68415-1-Ig)

    WB analysis of T-47D using 68415-1-Ig (same clone as 68415-1-PBS)

    T-47D cells were subjected to SDS PAGE followed by western blot with 68415-1-Ig (PNMA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68415-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using PNMA1 Monoclonal antibody (68415-1-Ig)

    IF Staining of PC-3 using 68415-1-Ig (same clone as 68415-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using PNMA1 antibody (68415-1-Ig, Clone: 2D8C6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68415-1-PBS in a different storage buffer formulation.

PNMA1 Monoclonal antibody, PBS Only

PNMA1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68415-1-PBS
Clone No.2D8C6

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Paraneoplastic antigen Ma1, MA1, 37 kDa neuronal protein, 2D8C6

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68415-1-PBS targets PNMA1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag33143

Product name: Recombinant human PNMA1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-353 aa of BC039577

Sequence: MAMTLLEDWCRGMDVNSQRALLVWGIPVNCDEAEIEETLQAAMPQVSYRMLGRMFWREENAKAALLELTGAVDYAAIPREMPGKGGVWKVLFKPPTSDAEFLERLHLFLAREGWTVQDVARVLGFQNPTPTPGPEMPAEMLNYILDNVIQPLVESIWYKRLTLFSGRDIPGPGEETFDPWLEHTNEVLEEWQVSDVEKRRRLMESLRGPAADVIRILKSNNPAITTAECLKALEQVFGSVESSRDAQIKFLNTYQNPGEKLSAYVIRLEPLLQKVVEKGAIDKDNVNQARLEQVIAGANHSGAIRRQLWLTGAGEGPAPNLFQLLVQIREEEAKEEEEEAEATLLQLGLEGHF

相同性解析による交差性が予測される生物種
Full Name paraneoplastic antigen MA1
Calculated molecular weight 353 aa, 40 kDa
Observed molecular weight37 kDa
GenBank accession numberBC039577
Gene Symbol PNMA1
Gene ID (NCBI) 9240
RRIDAB_3085131
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ8ND90
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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