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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using PNMA1 Monoclonal antibody (68415-1-Ig)

    WB analysis of PC-3 using 68415-1-Ig

    PC-3 cells were subjected to SDS PAGE followed by western blot with 68415-1-Ig (PNMA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of LNCaP cells using PNMA1 Monoclonal antibody (68415-1-Ig)

    WB analysis of LNCaP using 68415-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68415-1-Ig (PNMA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat testis tissue using PNMA1 Monoclonal antibody (68415-1-Ig)

    WB analysis of rat testis using 68415-1-Ig

    rat testis tissue were subjected to SDS PAGE followed by western blot with 68415-1-Ig (PNMA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of T-47D cells using PNMA1 Monoclonal antibody (68415-1-Ig)

    WB analysis of T-47D using 68415-1-Ig

    T-47D cells were subjected to SDS PAGE followed by western blot with 68415-1-Ig (PNMA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using PNMA1 Monoclonal antibody (68415-1-Ig)

    IF Staining of PC-3 using 68415-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using PNMA1 antibody (68415-1-Ig, Clone: 2D8C6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

PNMA1 Monoclonal antibody

PNMA1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68415-1-Ig
Clone No.2D8C6

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Paraneoplastic antigen Ma1, MA1, 37 kDa neuronal protein, 2D8C6

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inPC-3 cells, T-47D cells, LNCaP cells, rat testis tissue
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68415-1-Ig targets PNMA1 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag33143

Product name: Recombinant human PNMA1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-353 aa of BC039577

Sequence: MAMTLLEDWCRGMDVNSQRALLVWGIPVNCDEAEIEETLQAAMPQVSYRMLGRMFWREENAKAALLELTGAVDYAAIPREMPGKGGVWKVLFKPPTSDAEFLERLHLFLAREGWTVQDVARVLGFQNPTPTPGPEMPAEMLNYILDNVIQPLVESIWYKRLTLFSGRDIPGPGEETFDPWLEHTNEVLEEWQVSDVEKRRRLMESLRGPAADVIRILKSNNPAITTAECLKALEQVFGSVESSRDAQIKFLNTYQNPGEKLSAYVIRLEPLLQKVVEKGAIDKDNVNQARLEQVIAGANHSGAIRRQLWLTGAGEGPAPNLFQLLVQIREEEAKEEEEEAEATLLQLGLEGHF

相同性解析による交差性が予測される生物種
Full Name paraneoplastic antigen MA1
Calculated molecular weight 353 aa, 40 kDa
Observed molecular weight37 kDa
GenBank accession numberBC039577
Gene Symbol PNMA1
Gene ID (NCBI) 9240
RRIDAB_3085131
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ8ND90
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for PNMA1 antibody 68415-1-IgDownload protocol
WB protocol for PNMA1 antibody 68415-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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