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CoraLite® Plus 647 Anti-Mouse PLVAP (MECA-32) Rat IgG2a Recombinant Antibody

PLVAP Uni-rAbTM Recombinant Antibody for FC
Cat No. CL647-65664
Clone No.MECA-32

Host / Isotype

Rat / IgG2a

Reactivity

mouse

Applications

FC

MECA32, MECA-32, Panendothelial cell antigen, Plasmalemma vesicle protein 1, PV 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 647
Size/Concentration: 

-/ -


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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive FC detected inbEnd.3 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65664 targets PLVAP in FC applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rat / IgG2a
Class Recombinant
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name plasmalemma vesicle associated protein
GenBank accession numberBC013517
Gene Symbol Plvap
Gene ID (NCBI) 84094
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.09% sodium azide{{ptg:BufferTemp}}7.3
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Plasmalemma vesicle-associated protein (PLVAP), also known as PV-1, PAL-E and MECA-32 antigen, is an endothelial cell-specific type II membrane N-glycosylated glycoprotein with a molecular weight of approximately 50-65 kDa, that forms homodimers in situ (PMID: 7626790; 30231925). The MECA-32 antibody, derived from rats immunized with murine lymph node stroma, shows high specificity for mouse endothelium in both embryonic and mature tissues (PMID: 2683264; 7626790). It recognizes most endothelial cells in the embryonic and in the adult mouse, with the exception of the brain, skeletal, and cardiac muscle, where the MECA-32 antigen has a more restricted distribution. In skeletal and cardiac muscle only small arterioles and venules express the MECA-32 antigen, while in the brain its expression is negatively correlated with the differentiation of the vasculature to form the blood brain barrier (PMID: 7626790).

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 PLVAP antibody CL647-65664Download protocol
Standard Protocols
Click here to view our Standard Protocols
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