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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PLEKHA2 Polyclonal antibody (32169-1-AP)

    WB analysis using 32169-1-AP (same clone as 32169-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 32169-1-AP (PLEKHA2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32169-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using PLEKHA2 Polyclonal antibody (32169-1-AP)

    IF Staining of A431 using 32169-1-AP (same clone as 32169-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using PLEKHA2 antibody (32169-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 32169-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PLEKHA2 Polyclonal antibody (32169-1-AP)

    IF Staining of HeLa using 32169-1-AP (same clone as 32169-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PLEKHA2 antibody (32169-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 32169-1-PBS in a different storage buffer formulation.

PLEKHA2 Polyclonal antibody, PBS Only

PLEKHA2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 32169-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

TAPP-2, TAPP2, Tandem PH domain-containing protein 2, Pleckstrin homology domain-containing family A member 2, Pleckstrin Homology Domain Containing A2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

32169-1-PBS targets PLEKHA2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag36596

Product name: Recombinant human PLEKHA2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 101-205 aa of BC166639

Sequence: MKDWVEALNQASKITVPKGGGLPMTTEVLKSLAAPPALEKKPQVAYKTEIIGGVVVHTPISQNGGDGQEGSEPGSHTILRRSQSYIPTSGCRASTGPPLIKSGYC

相同性解析による交差性が予測される生物種
Full Name pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 2
Observed molecular weight42 kDa
GenBank accession numberBC166639
Gene Symbol PLEKHA2
Gene ID (NCBI) 59339
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9HB19
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PLEKHA2 (Pleckstrin homology domain containing A2), also known as TAPP2, is a member of the PLEKHA family of proteins.PLEKHA2 is a junctional protein with a Pleckstrin homology domain that is involved in signaling after lymphocyte activation and plays an important role in cytoskeletal reorganization driven by phosphatidylinositol 3-kinase.

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