Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, JurKat cells, RT-4 cells |
| Positive IF/ICC detected in | HeLa cells, A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32169-1-AP targets PLEKHA2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag36596 Product name: Recombinant human PLEKHA2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 101-205 aa of BC166639 Sequence: MKDWVEALNQASKITVPKGGGLPMTTEVLKSLAAPPALEKKPQVAYKTEIIGGVVVHTPISQNGGDGQEGSEPGSHTILRRSQSYIPTSGCRASTGPPLIKSGYC 相同性解析による交差性が予測される生物種 |
| Full Name | pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 2 |
| Observed molecular weight | 42 kDa |
| GenBank accession number | BC166639 |
| Gene Symbol | PLEKHA2 |
| Gene ID (NCBI) | 59339 |
| RRID | AB_3670213 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9HB19 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PLEKHA2 (Pleckstrin homology domain containing A2), also known as TAPP2, is a member of the PLEKHA family of proteins.PLEKHA2 is a junctional protein with a Pleckstrin homology domain that is involved in signaling after lymphocyte activation and plays an important role in cytoskeletal reorganization driven by phosphatidylinositol 3-kinase.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PLEKHA2 antibody 32169-1-AP | Download protocol |
| WB protocol for PLEKHA2 antibody 32169-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |