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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated PLEK Monoclonal antibody

PLEK Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1D5C2

Cat no : CL594-66431

Synonyms

FLJ27168, P47, Platelet 47 kDa protein, pleckstrin, PLEK

Validation Data Gallery

Applications:
  • FC experiment of HeLa using CL594-66431

    FC experiment of HeLa using CL594-66431

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human PLEK (CL594-66431, Clone:1D5C2) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66431-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL594-66431 targets PLEK in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PLEK fusion protein Ag3187 相同性解析による交差性が予測される生物種
Full Name pleckstrin
Calculated molecular weight 350 aa, 40 kDa
Observed molecular weight 40-47 kDa
GenBank accession numberBC018549
Gene symbol PLEK
Gene ID (NCBI) 5341
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLEK (pleckstrin; also known as p47) was originally identified as the major PKC substrate in platelets and later is found to be expressed in all cells of the hemopoietic system. Through two pleckstrin homology (PH) domains at its N and C termini, PLEK may interact with various protein and/or lipid ligands and serve as an intracellular adaptor/targeting protein. Predominantly cytosolic in unstimulated cells, PLEK would undergo transient redistribution to phagosomal membrane in response to stimuli. PLEK has been found to be hyperphosphorylated in diabetic mononuclear phagocytes and promote proinflammatory cytokine secretion in diabetes. (10477609, 17579087)

Protocols

Product Specific Protocols
FC protocol for CL594 PLEK antibody CL594-66431Download protocol
Standard Protocols
Click here to view our Standard Protocols