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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse brain tissue using PLCXD1 Polyclonal antibody (20161-1-AP)

    IHC staining of mouse brain using 20161-1-AP (same clone as 20161-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20161-1-AP (PLCXD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20161-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PLCXD1 Polyclonal antibody (20161-1-AP)

    IHC staining of mouse brain using 20161-1-AP (same clone as 20161-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20161-1-AP (PLCXD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20161-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using PLCXD1 Polyclonal antibody (20161-1-AP)

    IF Staining of A549 using 20161-1-AP (same clone as 20161-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using PLCXD1 antibody (20161-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20161-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using PLCXD1 Polyclonal antibody (20161-1-AP)

    IF Staining of U2OS using 20161-1-AP (same clone as 20161-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using PLCXD1 antibody (20161-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20161-1-PBS in a different storage buffer formulation.

PLCXD1 Polyclonal antibody, PBS Only

PLCXD1 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 20161-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

phosphatidylinositol phospholipase C, X domain containing 1, PI-PLC X domain-containing protein 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20161-1-PBS targets PLCXD1 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14086

Product name: Recombinant human PLCXD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-323 aa of BC005028

Sequence: MGGQVSASNSFSRLHCRNANEDWMSALCPRLWDVPLHHLSIPGSHDTMTYCLNKKSPISHEESRLLQLLNKALPCITRPVVLKWSVTQALDVTEQLDAGVRYLDLRIAHMLEGSEKNLHFVHMVYTTALVEDTLTEISEWLERHPREVVILACRNFEGLSEDLHEYLVACIKNIFGDMLCPRGEVPTLRQLWSRGQQVIVSYEDESSLRRHHELWPGVPYWWGNRVKTEALIRYLETMKSCGRPGGLFVAGINLTENLQYVLAHPSESLEKMTLPNLPRLSAWVREQCPGPGSRCTNIIAGDFIGADGFVSDVIALNQKLLWC

相同性解析による交差性が予測される生物種
Full Name phosphatidylinositol-specific phospholipase C, X domain containing 1
Calculated molecular weight 323 aa, 37 kDa
GenBank accession numberBC005028
Gene Symbol PLCXD1
Gene ID (NCBI) 55344
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NUJ7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Phosphatidylinositol-specific phospholipase C (PI-PLC) enzymes comprise a small family of receptor-regulated phosphodiesterases that control many cellular processes by the regulation of cytosolic calcium and/or the activity of several protein kinases. At least three tissue-specific PLCXD isoforms exist in humans, comprising hPLCXD-1, hPLCXD-2 and hPLCXD-3 (PMID: 22732399). Mutations in the PLCXD1 gene are associated with diseases such as Leri-Weill Dyschondrosteosis.

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