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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse brain tissue using PLCXD1 Polyclonal antibody (20161-1-AP)

    IHC staining of mouse brain using 20161-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20161-1-AP (PLCXD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PLCXD1 Polyclonal antibody (20161-1-AP)

    IHC staining of mouse brain using 20161-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20161-1-AP (PLCXD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using PLCXD1 Polyclonal antibody (20161-1-AP)

    IF Staining of A549 using 20161-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using PLCXD1 antibody (20161-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using PLCXD1 Polyclonal antibody (20161-1-AP)

    IF Staining of U2OS using 20161-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using PLCXD1 antibody (20161-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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PLCXD1 Polyclonal antibody

PLCXD1 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 20161-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Synonyms

phosphatidylinositol phospholipase C, X domain containing 1, PI-PLC X domain-containing protein 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells, U2OS cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20161-1-AP targets PLCXD1 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLCXD1 fusion protein Ag14086 相同性解析による交差性が予測される生物種
Full Name phosphatidylinositol-specific phospholipase C, X domain containing 1
Calculated molecular weight 323 aa, 37 kDa
GenBank accession numberBC005028
Gene Symbol PLCXD1
Gene ID (NCBI) 55344
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NUJ7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Phosphatidylinositol-specific phospholipase C (PI-PLC) enzymes comprise a small family of receptor-regulated phosphodiesterases that control many cellular processes by the regulation of cytosolic calcium and/or the activity of several protein kinases. At least three tissue-specific PLCXD isoforms exist in humans, comprising hPLCXD-1, hPLCXD-2 and hPLCXD-3 (PMID: 22732399). Mutations in the PLCXD1 gene are associated with diseases such as Leri-Weill Dyschondrosteosis.

Protocols

Product Specific Protocols
IHC protocol for PLCXD1 antibody 20161-1-APDownload protocol
IF protocol for PLCXD1 antibody 20161-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols