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Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using PKP4 Polyclonal antibody (14062-1-AP)

    WB analysis of A431 using 14062-1-AP (same clone as 14062-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 14062-1-AP (PKP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14062-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using PKP4 Polyclonal antibody (14062-1-AP)

    WB analysis of HepG2 using 14062-1-AP (same clone as 14062-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14062-1-AP (PKP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14062-1-PBS in a different storage buffer formulation.

PKP4 Polyclonal antibody, PBS Only

PKP4 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 14062-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14062-1-PBS targets PKP4 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5113

Product name: Recombinant human PKP4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 800-1149 aa of BC050308

Sequence: DQWDGVGPIPGLSKSPKGVEMLWHPSVVKPYLTLLAESSNPATLEGSAGSLQNLSAGNWKFAAYIRAAVRKEKGLPILVELLRMDNDRVVSSVATALRNMALDVRNKELIGKYAMRDLVNRLPGGNGPSVLSDETMAAICCALHEVTSKNMENAKALADSGGIEKLVNITKGRGDRSSLKVVKAAAQVLNTLWQYRDLRSIYKKDGWNQNHFITPVSTLERDRFKSHPSLSTTNQQMSPIIQSGSSKPSPIYISSYSSPAREQNRRLQHQQLYYSQDDSNRKNFDAYRLYLQSPHSYEDPYFDDRVHFPASTDYSTQYGLKSTTNYVDFYSTKRPSYRAEQYPGSPDSWV

相同性解析による交差性が予測される生物種
Full Name plakophilin 4
Calculated molecular weight 132 kDa
Observed molecular weight 125-140 kDa
GenBank accession numberBC050308
Gene Symbol PKP4
Gene ID (NCBI) 8502
RRIDAB_10640532
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99569
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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