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Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using PKP4 Polyclonal antibody (14062-1-AP)

    WB analysis of A431 using 14062-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 14062-1-AP (PKP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PKP4 Polyclonal antibody (14062-1-AP)

    WB analysis of HepG2 using 14062-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14062-1-AP (PKP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using PKP4 Polyclonal antibody (14062-1-AP)

    IHC staining of human stomach cancer using 14062-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14062-1-AP (PKP4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using PKP4 Polyclonal antibody (14062-1-AP)

    IHC staining of mouse stomach using 14062-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 14062-1-AP (PKP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat salivary gland tissue using PKP4 Polyclonal antibody (14062-1-AP)

    IHC staining of rat salivary gland using 14062-1-AP

    Immunohistochemical analysis of paraffin-embedded rat salivary gland tissue slide using 14062-1-AP (PKP4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat stomach tissue using PKP4 Polyclonal antibody (14062-1-AP)

    IHC staining of rat stomach using 14062-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 14062-1-AP (PKP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PKP4 Polyclonal antibody

PKP4 Polyclonal Antibody for WB, ELISA
Cat No. 14062-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

plakophilin 4, Plakophilin-4

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14062-1-AP targets PKP4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5113

Product name: Recombinant human PKP4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 800-1149 aa of BC050308

Sequence: DQWDGVGPIPGLSKSPKGVEMLWHPSVVKPYLTLLAESSNPATLEGSAGSLQNLSAGNWKFAAYIRAAVRKEKGLPILVELLRMDNDRVVSSVATALRNMALDVRNKELIGKYAMRDLVNRLPGGNGPSVLSDETMAAICCALHEVTSKNMENAKALADSGGIEKLVNITKGRGDRSSLKVVKAAAQVLNTLWQYRDLRSIYKKDGWNQNHFITPVSTLERDRFKSHPSLSTTNQQMSPIIQSGSSKPSPIYISSYSSPAREQNRRLQHQQLYYSQDDSNRKNFDAYRLYLQSPHSYEDPYFDDRVHFPASTDYSTQYGLKSTTNYVDFYSTKRPSYRAEQYPGSPDSWV

相同性解析による交差性が予測される生物種
Full Name plakophilin 4
Calculated molecular weight 132 kDa
Observed molecular weight 125-140 kDa
GenBank accession numberBC050308
Gene Symbol PKP4
Gene ID (NCBI) 8502
RRIDAB_10640532
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99569
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PKP4 antibody 14062-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem Biophys Res Commun

Hydrogen sulfide improves keratinocytes migration and diabetic skin wound healing via plakophilin 4.

Authors - Bishan Zhang
  • KO Validated
View Article
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