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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using PKC eta Polyclonal antibody (26916-1-AP)

    WB analysis of Jurkat using 26916-1-AP (same clone as 26916-1-PBS)

    WB result of PKC eta antibody (26916-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-PKC eta transfected Jurkat cells. This data was developed using the same antibody clone with 26916-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PKC eta Polyclonal antibody (26916-1-AP)

    WB analysis using 26916-1-AP (same clone as 26916-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 26916-1-AP (PKC eta antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26916-1-PBS in a different storage buffer formulation.

PKC eta Polyclonal antibody, PBS Only

PKC eta Polyclonal Antibody for WB, Indirect ELISA
Cat No. 26916-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

PRKCH, PKC L, PKCL, PRKCL, Protein kinase C eta type

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

26916-1-PBS targets PKC eta in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag25365

Product name: Recombinant human PRKCH protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 634-683 aa of BC037268

Sequence: RIKSREDVSNFDPDFIKEEPVLTPIDEGHLPMINQDEFRNFSYVSPELQP

相同性解析による交差性が予測される生物種
Full Name protein kinase C, eta
Observed molecular weight75-90 kDa
GenBank accession numberBC037268
Gene Symbol PRKCH
Gene ID (NCBI) 5583
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP24723
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PRKCH (gene ID: 5583) encodes protein kinase C eta (PKCη), a calcium‑independent, phospholipid‑dependent serine/threonine kinase of the novel PKC subfamily. It regulates cell proliferation, differentiation, and apoptosis, with prominent roles in keratinocyte differentiation, early B‑cell development, and epithelial barrier integrity. Genetic variants in PRKCH are linked to cerebral infarction and Alzheimer's disease, while PKCη exhibits context‑dependent functions in cancer-promoting progression and chemoresistance in some tumors (e.g., glioblastoma) but acting as a tumor suppressor in others (e.g., hepatocellular carcinoma).

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