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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using PKC eta Polyclonal antibody (26916-1-AP)

    WB analysis of Jurkat using 26916-1-AP

    WB result of PKC eta antibody (26916-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-PKC eta transfected Jurkat cells.

  • Western Blot (WB) analysis of various lysates using PKC eta Polyclonal antibody (26916-1-AP)

    WB analysis using 26916-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 26916-1-AP (PKC eta antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • KD/KO Validated

PKC eta Polyclonal antibody

PKC eta Polyclonal Antibody for WB, ELISA
Cat No. 26916-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

PRKCH, PKC L, PKCL, PRKCL, Protein kinase C eta type

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inPC-3 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26916-1-AP targets PKC eta in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag25365

Product name: Recombinant human PRKCH protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 634-683 aa of BC037268

Sequence: RIKSREDVSNFDPDFIKEEPVLTPIDEGHLPMINQDEFRNFSYVSPELQP

相同性解析による交差性が予測される生物種
Full Name protein kinase C, eta
Observed molecular weight75-90 kDa
GenBank accession numberBC037268
Gene Symbol PRKCH
Gene ID (NCBI) 5583
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP24723
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRKCH (gene ID: 5583) encodes protein kinase C eta (PKCη), a calcium‑independent, phospholipid‑dependent serine/threonine kinase of the novel PKC subfamily. It regulates cell proliferation, differentiation, and apoptosis, with prominent roles in keratinocyte differentiation, early B‑cell development, and epithelial barrier integrity. Genetic variants in PRKCH are linked to cerebral infarction and Alzheimer's disease, while PKCη exhibits context‑dependent functions in cancer-promoting progression and chemoresistance in some tumors (e.g., glioblastoma) but acting as a tumor suppressor in others (e.g., hepatocellular carcinoma).

Protocols

Product Specific Protocols
WB protocol for PKC eta antibody 26916-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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