Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 19132-1-AP (same clone as 19132-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 19132-1-AP (PKC Delta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.
WB analysis using 19132-1-AP (same clone as 19132-1-PBS)
Non-treated and PMA treated THP-1 cells were subjected to SDS PAGE followed by western blot with 29564-1-AP (Phospho-PKC delta (Thr507) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with PKC Delta antibody (19132-1-AP) subsequently. This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.
×
Non-treated and PMA treated THP-1 cells were subjected to SDS PAGE followed by western blot with 29564-1-AP (Phospho-PKC delta (Thr507) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with PKC Delta antibody (19132-1-AP) subsequently. This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.
WB analysis of mouse colon using 19132-1-AP (same clone as 19132-1-PBS)
mouse colon tissue were subjected to SDS PAGE followed by western blot with 19132-1-AP (PKC delta antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.
WB analysis of A431 using 19132-1-AP (same clone as 19132-1-PBS)
A431 cells were subjected to SDS PAGE followed by western blot with 19132-1-AP (PKC delta antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.
WB analysis of Jurkat using 19132-1-AP (same clone as 19132-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 19132-1-AP (PKC delta antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.
WB analysis of MCF-7 using 19132-1-AP (same clone as 19132-1-PBS)
MCF7 cells were subjected to SDS PAGE followed by western blot with 19132-1-AP (PKC delta antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.
WB analysis of HT-1080 using 19132-1-AP (same clone as 19132-1-PBS)
HT-1080 cells were subjected to SDS PAGE followed by western blot with 19132-1-AP (PKC delta antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.
IP experiment of A431 using 19132-1-AP (same clone as 19132-1-PBS)
IP result of anti-PKC Delta (IP:19132-1-AP, 4ug; Detection:19132-1-AP 1:800) with A431 cells lysate 2640 ug. This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.
IP experiment of A431 using 19132-1-AP (same clone as 19132-1-PBS)
IP result of anti-PKC Delta (IP:19132-1-AP, 4ug; Detection:19132-1-AP 1:500) with A431 cells lysate 2000ug. This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 19132-1-AP (same clone as 19132-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 19132-1-AP (PKC Delta antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 19132-1-AP (same clone as 19132-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 19132-1-AP (PKC Delta antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 19132-1-AP (same clone as 19132-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon using 19132-1-AP (PKC delta antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 19132-1-AP (same clone as 19132-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon using 19132-1-AP (PKC delta antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.
IF Staining of U2OS using 19132-1-AP (same clone as 19132-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PKC Delta antibody (19132-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.
IF Staining of U-251 using 19132-1-AP (same clone as 19132-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed U-251 cells using PKC Delta antibody (19132-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 19132-1-AP (same clone as 19132-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PKC Delta antibody (19132-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.
IF Staining of A431 using 19132-1-AP (same clone as 19132-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed A431 cells using PKC Delta antibody (19132-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.
FC experiment of HeLa using 19132-1-AP (same clone as 19132-1-PBS)
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PKC Delta (19132-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.
×
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PKC Delta (19132-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

PKC Delta Polyclonal antibody, PBS Only

PKC Delta Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Cat No. 19132-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

PRKCD, EC:2.7.10.2, EC:2.7.11.13, MAY1, nPKC-delta

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19132-1-PBS targets PKC Delta in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5631 Product name: Recombinant human PRKCD protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 326-676 aa of BC043350 Sequence: DMQDNSGTYGKIWEGSSKCNINNFIFHKVLGKGSFGKVLLGELKGRGEYFAIKALKKDVVLIDDDVECTMVEKRVLTLAAENPFLTHLICTFQTKDHLFFVMEFLNGGDLMYHIQDKGRFELYRATFYAAEIMCGLQFLHSKGIIYRDLKLDNVLLDRDGHIKIADFGMCKENIFGESRASTFCGTPDYIAPEILQGLKYTFSVDWWSFGVLLYEMLIGQSPFHGDDEDELFESIRVDTPHYPRWITKESKDILEKLFEREPTKRLGVTGNIKIHPFFKTINWTLLEKRRLEPPFRPKVKSPRDYSNFDQEFLNEKARLSYSDKNLIDSMDQSAFAGFSFVNPKFEHLLED 相同性解析による交差性が予測される生物種
Full Name	protein kinase C, delta
Calculated molecular weight	78 kDa
Observed molecular weight	70 kDa
GenBank accession number	BC043350
Gene Symbol	PKC Delta
Gene ID (NCBI)	5580
RRID	AB_10637263
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q05655
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 19132-1-AP (same clone as 19132-1-PBS)

WB analysis using 19132-1-AP (same clone as 19132-1-PBS)

WB analysis of mouse colon using 19132-1-AP (same clone as 19132-1-PBS)

WB analysis of A431 using 19132-1-AP (same clone as 19132-1-PBS)

WB analysis of Jurkat using 19132-1-AP (same clone as 19132-1-PBS)

WB analysis of MCF-7 using 19132-1-AP (same clone as 19132-1-PBS)

WB analysis of HT-1080 using 19132-1-AP (same clone as 19132-1-PBS)

IP experiment of A431 using 19132-1-AP (same clone as 19132-1-PBS)

IP experiment of A431 using 19132-1-AP (same clone as 19132-1-PBS)

IHC staining of mouse liver using 19132-1-AP (same clone as 19132-1-PBS)

IHC staining of mouse liver using 19132-1-AP (same clone as 19132-1-PBS)

IHC staining of human colon using 19132-1-AP (same clone as 19132-1-PBS)

IHC staining of human colon using 19132-1-AP (same clone as 19132-1-PBS)

IF Staining of U2OS using 19132-1-AP (same clone as 19132-1-PBS)

IF Staining of U-251 using 19132-1-AP (same clone as 19132-1-PBS)

IF Staining of HeLa using 19132-1-AP (same clone as 19132-1-PBS)

IF Staining of A431 using 19132-1-AP (same clone as 19132-1-PBS)

FC experiment of HeLa using 19132-1-AP (same clone as 19132-1-PBS)