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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PIK3C2A Recombinant monoclonal antibody (88380-1-RR)

    WB analysis using 88380-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 88380-1-RR (PIK3C2A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PIK3C2A Recombinant monoclonal antibody (88380-1-RR)

    IF Staining of HeLa using 88380-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PIK3C2A antibody (88380-1-RR, Clone: 260864A5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

PIK3C2A Recombinant monoclonal antibody

PIK3C2A Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 88380-1-RR
Clone No.260864A5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

EC:2.7.1.137, Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha, Phosphoinositide 3-kinase-C2-alpha, PI3 K C2A, PI3K C2 alpha

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, PC-3 cells, NIH/3T3 cells, C6 cells, mouse testis tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

88380-1-RR targets PIK3C2A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag16894

Product name: Recombinant human PIK3C2A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-338 aa of BC113658

Sequence: MAQISSNSGFKECPSSHPEPTRAKDVDKEEALQMEAEALAKLQKDRQVTDNQRGFELSSSTRKKAQVYNKQDYDLMVFPESDSQKRALDIDVEKLTQAELEKLLLDDSFETKKTPVLPVTPILSPSFSAQLYFRPTIQRGQWPPGLPGPSTYALPSIYPSTYSKQAAFQNGFNPRMPTFPSTEPIYLSLPGQSPYFSYPLTPATPFHPQGSLPIYRPVVSTDMAKLFDKIASTSEFLKNGKARTDLEITDSKVSNLQVSPKSEDISKFDWLDLDPLSKPKVDNVEVLDHEEEKNVSSLLAKDPWDAVLLEERSTANCHLERKVNGKSLSVATVTRSQS

相同性解析による交差性が予測される生物種
Full Name phosphoinositide-3-kinase, class 2, alpha polypeptide
Calculated molecular weight 1686 aa, 191 kDa
Observed molecular weight 191 kDa
GenBank accession numberBC113658
Gene Symbol PIK3C2A
Gene ID (NCBI) 5286
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO00443
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PIK3C2A, also known as PI3K-C2alpha, is a member of phosphatidylinositol -4- phosphate 3- kinase (PI3K) family. Genes in this family play a key role in cell signal transduction. The main function of PIK3C2A is to participate in the metabolism of phosphatidylinositol in cells, specifically, it is responsible for converting phosphatidylinositol -4- phosphate (PIP) into phosphatidylinositol -3,4- diphosphate (PIP2).

Protocols

Product Specific Protocols
IF protocol for PIK3C2A antibody 88380-1-RRDownload protocol
WB protocol for PIK3C2A antibody 88380-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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