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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PIK3C2A Recombinant monoclonal antibody (88380-1-RR)

    WB analysis using 88380-1-RR (same clone as 88380-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 88380-1-RR (PIK3C2A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88380-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP03538-1

    Sandwich ELISA sample test of MP03538-1

    The mean PIK3C2A concentration was determined to be 3.1 ng/mL in THP-1 cell extract based on a 1.2 mg/mL extract load, 4.0 ng/mL in MG-63 cell extract based on a 1.2 mg/mL extract load and 3.4 ng/mL in HL-60 cell extract based on a 1.2 mg/mL extract load.

  • Sandwich ELISA sample test of MP03538-1

    Sandwich ELISA sample test of MP03538-1

    The mean PIK3C2A concentration was determined to be 16.3 ng/mL in HT-29 cell extract based on a 1.2 mg/mL extract load, 7.3 ng/mL in A431 cell extract based on a 1.2 mg/mL extract load and 10.5 ng/mL in RT-4 cell extract based on a 1.2 mg/mL extract load.

  • Sandwich ELISA sample test of MP03538-1

    Sandwich ELISA sample test of MP03538-1

    The mean PIK3C2A concentration was determined to be 11.0 ng/mL in HaCaT cell extract based on a 1.2 mg/mL extract load, 9.6 ng/mL in PC-3 cell extract based on a 1.2 mg/mL extract load and 3.4 ng/mL in HT-1080 cell extract based on a 1.2 mg/mL extract load.

  • Sandwich ELISA standard curve of MP03538-1

    Sandwich ELISA standard curve of MP03538-1

    Sandwich ELISA standard curve of MP03538-1, Human PIK3C2A Recombinant Matched Antibody Pair - PBS only. 88380-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag16894. 88380-3-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PIK3C2A Recombinant monoclonal antibody (88380-1-RR)

    IF Staining of HeLa using 88380-1-RR (same clone as 88380-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PIK3C2A antibody (88380-1-RR, Clone: 260864A5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 88380-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP03538-1

    Cytometric bead array sample test of MP03538-1

    The mean PIK3C2A concentration was determined to be 10.2 ng/mL in Caco-2 cell extract based on a 1.2 mg/mL extract load, 15.5 ng/mL in HT-29 cell extract based on a 1.2 mg/mL extract load, 3.0 ng/mL in HL-60 cell extract based on a 1.2 mg/mL extract load.

  • Cytometric bead array standard curve of MP03538-1

    Cytometric bead array standard curve of MP03538-1

    Cytometric bead array standard curve of MP03538-1, PIK3C2A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 88380-1-PBS. Detection antibody: 88380-3-PBS. Standard: Ag16894. Range: 0.156-20 ng/mL.

PIK3C2A Recombinant monoclonal antibody, PBS Only (Capture)

PIK3C2A Uni-rAb® Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA
Cat No. 88380-1-PBS
Clone No.260864A5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

EC:2.7.1.137, Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha, Phosphoinositide 3-kinase-C2-alpha, PI3 K C2A, PI3K C2 alpha

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

88380-1-PBS targets PIK3C2A as part of a matched antibody pair:

MP03538-1: 88380-1-PBS capture and 88380-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag16894

Product name: Recombinant human PIK3C2A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-338 aa of BC113658

Sequence: MAQISSNSGFKECPSSHPEPTRAKDVDKEEALQMEAEALAKLQKDRQVTDNQRGFELSSSTRKKAQVYNKQDYDLMVFPESDSQKRALDIDVEKLTQAELEKLLLDDSFETKKTPVLPVTPILSPSFSAQLYFRPTIQRGQWPPGLPGPSTYALPSIYPSTYSKQAAFQNGFNPRMPTFPSTEPIYLSLPGQSPYFSYPLTPATPFHPQGSLPIYRPVVSTDMAKLFDKIASTSEFLKNGKARTDLEITDSKVSNLQVSPKSEDISKFDWLDLDPLSKPKVDNVEVLDHEEEKNVSSLLAKDPWDAVLLEERSTANCHLERKVNGKSLSVATVTRSQS

相同性解析による交差性が予測される生物種
Full Name phosphoinositide-3-kinase, class 2, alpha polypeptide
Calculated molecular weight 1686 aa, 191 kDa
Observed molecular weight 191 kDa
GenBank accession numberBC113658
Gene Symbol PIK3C2A
Gene ID (NCBI) 5286
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO00443
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PIK3C2A, also known as PI3K-C2alpha, is a member of phosphatidylinositol -4- phosphate 3- kinase (PI3K) family. Genes in this family play a key role in cell signal transduction. The main function of PIK3C2A is to participate in the metabolism of phosphatidylinositol in cells, specifically, it is responsible for converting phosphatidylinositol -4- phosphate (PIP) into phosphatidylinositol -3,4- diphosphate (PIP2).

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