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Validation Data Gallery

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  • Western Blot (WB) analysis of Neuro-2a cells using PHOX2B Monoclonal antibody (66254-1-Ig)

    WB analysis of Neuro-2a using 66254-1-Ig (same clone as 66254-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66254-1-Ig (PHOX2B Antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66254-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of neuroblastoma tissue using PHOX2B Monoclonal antibody (66254-1-Ig)

    IHC staining of neuroblastoma using 66254-1-Ig (same clone as 66254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded neuroblastoma tissue slide using 66254-1-Ig (PHOX2B antibody) at dilution of 1:300 (under 40x lens). This data was developed using the same antibody clone with 66254-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of neuroblastoma tissue using PHOX2B Monoclonal antibody (66254-1-Ig)

    IHC staining of neuroblastoma using 66254-1-Ig (same clone as 66254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded neuroblastoma tissue slide using 66254-1-Ig (PHOX2B antibody) at dilution of 1:300 (under 10x lens). This data was developed using the same antibody clone with 66254-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Neuro-2a cells using PHOX2B Monoclonal antibody (66254-1-Ig)

    IF Staining of Neuro-2a using 66254-1-Ig (same clone as 66254-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using PHOX2B antibody (66254-1-Ig, Clone: 1F5F5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66254-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using PHOX2B Monoclonal antibody (66254-1-Ig)

    IF Staining of SH-SY5Y using 66254-1-Ig (same clone as 66254-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using PHOX2B antibody (66254-1-Ig, Clone: 1F5F5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66254-1-PBS in a different storage buffer formulation.

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PHOX2B Monoclonal antibody, PBS Only

PHOX2B Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66254-1-PBS
Clone No.1F5F5

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

NBPhox, Neuroblastoma Phox, paired like homeobox 2b, PHOX2B, PHOX2B homeodomain protein, PMX2B

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1 mg/ml
100 μg, 1 mg/ml

¥167,000/ 100 μg

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66254-1-PBS targets PHOX2B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PHOX2B fusion protein Ag13196 相同性解析による交差性が予測される生物種
Full Name paired-like homeobox 2b
Calculated molecular weight 31 kDa
Observed molecular weight 32-36 kDa
GenBank accession numberBC017199
Gene Symbol PHOX2B
Gene ID (NCBI) 8929
RRIDAB_2881642
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99453
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Paired mesoderm homeobox protein 2B (PHOX2B), also named as Neuroblastoma Phox, is a 314 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the paired homeobox family. PHOX2B localizes in the nucleus and is expressed in neuroblastoma, brain and adrenal gland. PHOX2B plays a role in the development of several major noradrenergic neuron populations, including the locus coeruleus. PHOX2B has an association with congenital central hypoventilation syndrome.