Validation Data Gallery
Tested Applications
| Positive WB detected in | Neuro-2a cells |
| Positive IHC detected in | neuroblastoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells, Neuro-2a cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 3 publications below |
Product Information
66254-1-Ig targets PHOX2B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13196 Product name: Recombinant human PHOX2B protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 63-314 aa of BC017199 Sequence: GSCSLGTLRDHQSSPYAAVPYKLFTDHGGLNEKRKQRRIRTTFTSAQLKELERVFAETHYPDIYTREELALKIDLTEARVQVWFQNRRAKFRKQERAAAAAAAAAKNGSSGKKSDSSRDDESKEAKSTDPDSTGGPGPNPNPTPSCGANGGGGGGPSPAGAPGAAGPGGPGGEPGKGGAAAAAAAAAAAAAAAAAAAAGGLAAAGGPGQGWAPGPGPITSIPDSLGGPFASVLSSLQRPNGAKAALVKSSMF 相同性解析による交差性が予測される生物種 |
| Full Name | paired-like homeobox 2b |
| Calculated molecular weight | 31 kDa |
| Observed molecular weight | 32-36 kDa |
| GenBank accession number | BC017199 |
| Gene Symbol | PHOX2B |
| Gene ID (NCBI) | 8929 |
| RRID | AB_2881642 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q99453 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Paired mesoderm homeobox protein 2B (PHOX2B), also named as Neuroblastoma Phox, is a 314 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the paired homeobox family. PHOX2B localizes in the nucleus and is expressed in neuroblastoma, brain and adrenal gland. PHOX2B plays a role in the development of several major noradrenergic neuron populations, including the locus coeruleus. PHOX2B has an association with congenital central hypoventilation syndrome.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PHOX2B antibody 66254-1-Ig | Download protocol |
| IHC protocol for PHOX2B antibody 66254-1-Ig | Download protocol |
| WB protocol for PHOX2B antibody 66254-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Signal Mitotic cell responses to substrate topological cues are independent of the molecular nature of adhesion. | ||
Nat Biotechnol Generation of locus coeruleus norepinephrine neurons from human pluripotent stem cells | ||
Dev Growth Differ Chromosomal localization of PHOX2B during M-phase is disrupted in disease-associated mutants |
