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Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using PHLDB2 Polyclonal antibody (27940-1-AP)

    WB analysis of A431 using 27940-1-AP (same clone as 27940-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 27940-1-AP (PHLDB2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27940-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PHLDB2 Polyclonal antibody (27940-1-AP)

    IHC staining of human placenta using 27940-1-AP (same clone as 27940-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27940-1-AP (PHLDB2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27940-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PHLDB2 Polyclonal antibody (27940-1-AP)

    IHC staining of human placenta using 27940-1-AP (same clone as 27940-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27940-1-AP (PHLDB2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27940-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using PHLDB2 Polyclonal antibody (27940-1-AP)

    IHC staining of mouse heart using 27940-1-AP (same clone as 27940-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27940-1-AP (PHLDB2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27940-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using PHLDB2 Polyclonal antibody (27940-1-AP)

    IHC staining of mouse heart using 27940-1-AP (same clone as 27940-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27940-1-AP (PHLDB2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27940-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using PHLDB2 Polyclonal antibody (27940-1-AP)

    IHC staining of human skin cancer using 27940-1-AP (same clone as 27940-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 27940-1-AP (PHLDB2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27940-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using PHLDB2 Polyclonal antibody (27940-1-AP)

    IHC staining of human skin cancer using 27940-1-AP (same clone as 27940-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 27940-1-AP (PHLDB2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27940-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using PHLDB2 Polyclonal antibody (27940-1-AP)

    IHC staining of human skin cancer using 27940-1-AP (same clone as 27940-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 27940-1-AP (PHLDB2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27940-1-PBS in a different storage buffer formulation.

PHLDB2 Polyclonal antibody, PBS Only

PHLDB2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 27940-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Protein LL5-beta, Pleckstrin homology-like domain family B member 2, LL5beta, LL5B

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27940-1-PBS targets PHLDB2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag27331

Product name: Recombinant human PHLDB2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-350 aa of BC142678

Sequence: MEEHSYIQKELDLQNGSLEEDSVVHSVENDSQNMMESLSPKKYSSSLRFKANGDYSGSYLTLSQPVPAKRSPSPLGTSVRSSPSLAKIQGSKQFSYDGTDKNIPMKPPTPLLNTTSSLSGYPLGRADFDHYTGRDSERALRLSEKPPYSKYSSRHKSHDNVYSLGGLEGRKASGSLLAMWNGSSLSDAGPPPISRSGAASMPSSPKQARKMSIQDSLALQPKLTRHKELASENINLRTRKYSSSSLSHMGAYSRSLPRLYRATENQLTPLSLPPRNSLGNSKRTKLGEKDLPHSVIDNDNYLNFSSLSSGALPYKTSASEGNPYVSSTLSVPASPRVARKMLLASTSSCA

相同性解析による交差性が予測される生物種
Full Name pleckstrin homology-like domain, family B, member 2
Calculated molecular weight 1253 aa, 142 kDa
Observed molecular weight150 kDa
GenBank accession numberBC142678
Gene Symbol PHLDB2
Gene ID (NCBI) 90102
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86SQ0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The pleckstrin homology-like domain (PHLD) class of proteins are multifunctional proteins comprised of two families of proteins, PHLDA and PHLDB, each with 3 members: PHLDA1, PHLDA2, PHDLA3, PHLDB1, PHLDB2, and PHLDB3 (PMID: 32429563). All the family members possess a pleckstrin homology (PH) domain. PHLB2, also known as LL5-beta, contains two predicted coiled-coil domains, a serine-rich region predicted to contain multiple protein kinase C phosphorylation sites in addition to a PH domain (PMID: 15851520). PHLB2 is a filamin-binding protein that is an effector of the PI3K signaling pathway (PMID: 17174070). It is a PtdIns(3,4,5)P3 (PIP3) binding protein and can translocate to the plasma membrane in a receptor and PI3K-dependant manner (PMID: 12376540). PHLB2 has also been reported as a component of the postsynaptic apparatus and a regulator of postsynaptic differentiation (PMID: 15851520).

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