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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PHLDB2 Polyclonal antibody

PHLDB2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27940-1-AP

Synonyms

LL5b, LL5beta, PHLDB2, Protein LL5 beta

Validation Data Gallery

Applications:
  • WB analysis of A431 using 27940-1-AP

    WB analysis of A431 using 27940-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 27940-1-AP (PHLDB2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Sample-dependent, check data in validation data gallery

Product Information

27940-1-AP targets PHLDB2 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHLDB2 fusion protein Ag27331 相同性解析による交差性が予測される生物種
Full Name pleckstrin homology-like domain, family B, member 2
Calculated molecular weight 1253 aa, 142 kDa
Observed molecular weight150 kDa
GenBank accession numberBC142678
Gene symbol PHLDB2
Gene ID (NCBI) 90102
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The pleckstrin homology-like domain (PHLD) class of proteins are multifunctional proteins comprised of two families of proteins, PHLDA and PHLDB, each with 3 members: PHLDA1, PHLDA2, PHDLA3, PHLDB1, PHLDB2, and PHLDB3 (PMID: 32429563). All the family members possess a pleckstrin homology (PH) domain. PHLB2, also known as LL5-beta, contains two predicted coiled-coil domains, a serine-rich region predicted to contain multiple protein kinase C phosphorylation sites in addition to a PH domain (PMID: 15851520). PHLB2 is a filamin-binding protein that is an effector of the PI3K signaling pathway (PMID: 17174070). It is a PtdIns(3,4,5)P3 (PIP3) binding protein and can translocate to the plasma membrane in a receptor and PI3K-dependant manner (PMID: 12376540). PHLB2 has also been reported as a component of the postsynaptic apparatus and a regulator of postsynaptic differentiation (PMID: 15851520).

Protocols

Product Specific Protocols
WB protocol for PHLDB2 antibody 27940-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols