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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PHLDB1 Polyclonal antibody (33225-1-AP)

    WB analysis using 33225-1-AP (same clone as 33225-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 33225-1-AP (PHLDB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 33225-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PHLDB1 Polyclonal antibody (33225-1-AP)

    IHC staining of human ovary tumor using 33225-1-AP (same clone as 33225-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 33225-1-AP (PHLDB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33225-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PHLDB1 Polyclonal antibody (33225-1-AP)

    IHC staining of human ovary tumor using 33225-1-AP (same clone as 33225-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 33225-1-AP (PHLDB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33225-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using PHLDB1 Polyclonal antibody (33225-1-AP)

    IHC staining of mouse heart using 33225-1-AP (same clone as 33225-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 33225-1-AP (PHLDB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33225-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using PHLDB1 Polyclonal antibody (33225-1-AP)

    IF Staining of U2OS using 33225-1-AP (same clone as 33225-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using PHLDB1 antibody (33225-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 33225-1-PBS in a different storage buffer formulation.

PHLDB1 Polyclonal antibody, PBS Only

PHLDB1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 33225-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

DLNB07, KIAA0638, LL5A, Pleckstrin Homology Like Domain Family B Member 1, Pleckstrin homology-like domain family B member 1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

33225-1-PBS targets PHLDB1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag37254

Product name: Recombinant human PHLDB1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 620-800 aa of BC098586

Sequence: LCAEYSRADGGPEAGELPSIGEATAALALAGRRPSRGLAGASGRSSEEPGVATQRLWESMERSDEENLKEECSSTESTQQEHEDAPSTKLQGEVLALEEERAQVLGHVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVALETGIQKERDKEAEALET

相同性解析による交差性が予測される生物種
Full Name pleckstrin homology-like domain, family B, member 1
Observed molecular weight151 kDa
GenBank accession numberBC098586
Gene Symbol PHLDB1
Gene ID (NCBI) 23187
RRIDAB_3742903
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ86UU1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PHLDB1 (Pleckstrin Homology-like Domain Family B Member 1), also known as LL5α, is a scaffolding protein that specifically binds to phosphatidylinositol-3,4,5-trisphosphate (PIP3) through its C-terminal PH domain, anchoring microtubule plus ends to the cell cortex. Functionally, PHLDB1 acts as an insulin-responsive protein that translocates from the cytoplasm to the plasma membrane upon insulin stimulation, promoting GLUT4 translocation and glucose uptake by enhancing Akt phosphorylation. In addition, PHLDB1 plays a key role in cell migration. Studies have shown that its genetic polymorphisms are associated with glioma risk, and by enhancing PI3K/AKT signaling, it promotes tumor cell invasion and metastasis, making it a potential prognostic marker and therapeutic target.

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