Validation Data Gallery
Tested Applications
| Positive WB detected in | K-562 cells, MG-63 cells, U2OS cells |
| Positive IHC detected in | human ovary tumor tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33225-1-AP targets PHLDB1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag37254 Product name: Recombinant human PHLDB1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 620-800 aa of BC098586 Sequence: LCAEYSRADGGPEAGELPSIGEATAALALAGRRPSRGLAGASGRSSEEPGVATQRLWESMERSDEENLKEECSSTESTQQEHEDAPSTKLQGEVLALEEERAQVLGHVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVALETGIQKERDKEAEALET 相同性解析による交差性が予測される生物種 |
| Full Name | pleckstrin homology-like domain, family B, member 1 |
| Observed molecular weight | 151 kDa |
| GenBank accession number | BC098586 |
| Gene Symbol | PHLDB1 |
| Gene ID (NCBI) | 23187 |
| RRID | AB_3742903 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q86UU1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PHLDB1 (Pleckstrin Homology-like Domain Family B Member 1), also known as LL5α, is a scaffolding protein that specifically binds to phosphatidylinositol-3,4,5-trisphosphate (PIP3) through its C-terminal PH domain, anchoring microtubule plus ends to the cell cortex. Functionally, PHLDB1 acts as an insulin-responsive protein that translocates from the cytoplasm to the plasma membrane upon insulin stimulation, promoting GLUT4 translocation and glucose uptake by enhancing Akt phosphorylation. In addition, PHLDB1 plays a key role in cell migration. Studies have shown that its genetic polymorphisms are associated with glioma risk, and by enhancing PI3K/AKT signaling, it promotes tumor cell invasion and metastasis, making it a potential prognostic marker and therapeutic target.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PHLDB1 antibody 33225-1-AP | Download protocol |
| IHC protocol for PHLDB1 antibody 33225-1-AP | Download protocol |
| WB protocol for PHLDB1 antibody 33225-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |