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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated PHIP Polyclonal antibody

PHIP Polyclonal Antibody for IF, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-20933

Synonyms

ndrp, PH interacting protein, PHIP

Validation Data Gallery

Applications:
  • IF Staining of MCF-7 using CL488-20933

    IF Staining of MCF-7 using CL488-20933

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 PHIP antibody (CL488-20933) at dilution of 1:200, CL594-phalloidin (red).

  • FC experiment of MCF-7 using CL488-20933

    FC experiment of MCF-7 using CL488-20933

    1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated PHIP Polyclonal antibody (CL488-20933)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF detected inMCF-7 cells
Positive FC detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 20933-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-20933 targets PHIP in IF, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHIP fusion protein Ag14968 相同性解析による交差性が予測される生物種
Full Name pleckstrin homology domain interacting protein
Calculated molecular weight 1821 aa, 207 kDa
Observed molecular weight 207 kDa
GenBank accession numberBC008909
Gene symbol PHIP
Gene ID (NCBI) 55023
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PHIP is a protein that binds to the insulin receptor substrate 1 protein and regulates glucose transporter translocation in skeletal muscle cells. PHIP may also regulate growth and survival of pancreatic beta cells. Elevated copy number of this gene may be associated with melanoma severity and the encoded protein may promote melanoma metastasis in human patients.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 PHIP antibody CL488-20933Download protocol
FC protocol for CL Plus 488 PHIP antibody CL488-20933Download protocol
Standard Protocols
Click here to view our Standard Protocols