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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PHGDH Monoclonal antibody (67591-1-Ig)

    WB analysis using 67591-1-Ig (same clone as 67591-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67591-1-Ig (PHGDH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67591-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using PHGDH Monoclonal antibody (67591-1-Ig)

    IP experiment of HeLa using 67591-1-Ig (same clone as 67591-1-PBS)

    IP result of anti-PHGDH (IP:67591-1-Ig, 4ug; Detection:67591-1-Ig 1:3000) with HeLa cells lysate 980 ug. This data was developed using the same antibody clone with 67591-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using PHGDH Monoclonal antibody (67591-1-Ig)

    IHC staining of human urothelial carcinoma using 67591-1-Ig (same clone as 67591-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67591-1-Ig (PHGDH antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67591-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PHGDH Monoclonal antibody (67591-1-Ig)

    IF Staining of HeLa using 67591-1-Ig (same clone as 67591-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PHGDH antibody (67591-1-Ig, Clone: 1E8B8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67591-1-PBS in a different storage buffer formulation.

PHGDH Monoclonal antibody, PBS Only

PHGDH Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 67591-1-PBS
Clone No.1E8B8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

EC:1.1.1.37, D-3-phosphoglycerate dehydrogenase, 3-PGDH, 3PGDH, 3 PGDH

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67591-1-PBS targets PHGDH in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag6877

Product name: Recombinant human PHGDH protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 186-533 aa of BC000303

Sequence: FGVQQLPLEEIWPLCDFITVHTPLLPSTTGLLNDNTFAQCKKGVRVVNCARGGIVDEGALLRALQSGQCAGAALDVFTEEPPRDRALVDHENVISCPHLGASTKEAQSRCGEEIAVQFVDMVKGKSLTGVVNAQALTSAFSPHTKPWIGLAEALGTLMRAWAGSPKGTIQVITQGTSLKNAGNCLSPAVIVGLLKEASKQADVNLVNAKLLVKEAGLNVTTSHSPAAPGEQGFGECLLAVALAGAPYQAVGLVQGTTPVLQGLNGAVFRPEVPLRRDLPLLLFRTQTSDPAMLPTMIGLLAEAGVRLLSYQTSLVSDGETWHVMGISSLLPSLEAWKQHVTEAFQFHF

相同性解析による交差性が予測される生物種
Full Name phosphoglycerate dehydrogenase
Calculated molecular weight 57 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC000303
Gene Symbol PHGDH
Gene ID (NCBI) 26227
RRIDAB_2882799
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDO43175
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PHGDH(D-3-phosphoglycerate dehydrogenase) is also named as 3-PGDH, PGDH3 and belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. It catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor. 3-PGDH deficiency is a rare recessive inborn error in the biosynthesis of the amino acid L-serine characterized clinically by congenital microcephaly, psychomotor retardation, and intractable seizures(PMID:19235232 ).

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