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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PGRMC2 Monoclonal antibody

PGRMC2 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

5F10D7

Cat no : CL488-60249

Synonyms

DG6, PGRMC2, PMBP

Validation Data Gallery

Applications:
  • FC experiment of HepG2 using CL488-60249

    FC experiment of HepG2 using CL488-60249

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human PGRMC2 (CL488-60249, Clone:5F10D7) (red), or 0.8 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PGRMC2 Ready-To-Use IHC Kit. PGRMC2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60249-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-60249 targets PGRMC2 in FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PGRMC2 fusion protein Ag20077 相同性解析による交差性が予測される生物種
Full Name progesterone receptor membrane component 2
Calculated molecular weight 223 aa, 24 kDa
Observed molecular weight 24+28 kDa
GenBank accession numberBC016692
Gene symbol PGRMC2
Gene ID (NCBI) 10424
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Membrane-associated progesterone receptor component 2 (PGRMC2), also known as DG6 or PMBP, belongs to the cytochrome b5 family. This protein might play a role in cancer. The gene of PGRMC2 maps to chromosome 4q26, and encodes a 223-amino acid single-pass membrane protein with a cytochrome b5 heme-binding domain in its cytoplasmic domain. PGRMC2 has a calculated molecular weight of 24 kDa. The band of about 28 kDa detected by this monoclonal antibody is unknown but may represent phosphorylated form of PGRMC2 (PMID: 28104494).

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 PGRMC2 antibody CL488-60249Download protocol
Standard Protocols
Click here to view our Standard Protocols