Validation Data Gallery

Filter: AllWBIHC

WB analysis using 15550-1-AP (same clone as 15550-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 15550-1-AP (PGAM2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15550-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 15550-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15550-1-AP (PGAM2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 15550-1-AP (same clone as 15550-1-PBS)
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15550-1-AP (PGAM2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15550-1-PBS in a different storage buffer formulation.
WB analysis of mouse skeletal muscle using 15550-1-AP (same clone as 15550-1-PBS)
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15550-1-AP (PGAM2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15550-1-PBS in a different storage buffer formulation.
WB analysis of mouse heart using 15550-1-AP (same clone as 15550-1-PBS)
mouse heart tissue were subjected to SDS PAGE followed by western blot with 15550-1-AP (PGAM2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15550-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 15550-1-AP (same clone as 15550-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15550-1-AP (PGAM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15550-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 15550-1-AP (same clone as 15550-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15550-1-AP (PGAM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15550-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 15550-1-AP (same clone as 15550-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15550-1-AP (PGAM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15550-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 15550-1-AP (same clone as 15550-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15550-1-AP (PGAM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15550-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 15550-1-AP (same clone as 15550-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15550-1-AP (PGAM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15550-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 15550-1-AP (same clone as 15550-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15550-1-AP (PGAM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15550-1-PBS in a different storage buffer formulation.
IHC staining of mouse skeletal muscle using 15550-1-AP (same clone as 15550-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15550-1-AP (PGAM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15550-1-PBS in a different storage buffer formulation.

PGAM2 Polyclonal antibody, PBS Only

PGAM2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15550-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

BPG dependent PGAM 2, BPG-dependent PGAM 2, EC:5.4.2.11, Muscle-specific phosphoglycerate mutase, PGAM M

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15550-1-PBS targets PGAM2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7908 Product name: Recombinant human PGAM2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-253 aa of BC001904 Sequence: MATHRLVMVRHGESTWNQENRFCGWFDAELSEKGTEEAKRGAKAIKDAKMEFDICYTSVLKRAIRTLWAILDGTDQMWLPVVRTWRLNERHYGGLTGLNKAETAAKHGEEQVKIWRRSFDIPPPPMDEKHPYYNSISKERRYAGLKPGELPTCESLKDTIARALPFWNEEIVPQIKAGKRVLIAAHGNSLRGIVKHLEGMSDQAIMELNLPTGIPIVYELNKELKPTKPMQFLGDEETVRKAMEAVAAQGKAK 相同性解析による交差性が予測される生物種
Full Name	phosphoglycerate mutase 2 (muscle)
Calculated molecular weight	29 kDa
Observed molecular weight	29 kDa
GenBank accession number	BC001904
Gene Symbol	PGAM2
Gene ID (NCBI)	5224
RRID	AB_2299336
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P15259
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

Phosphoglycerate mutase (PGAM), an important enzyme in the glycolytic pathway, catalyzes the transfer of a phosphate group between the 2 and the 3 positions of glyceric acid. The muscle-specific isoform (type M, PGAM2) of phosphoglycerate mutase (PGAM) is a housekeeping enzyme and it catalyzes the conversion of 3-phosphoglycerate into 2-phosphoglycerate in the glycolysis process to release energy. It is encoded by the Pgam2 gene. In addition, it is demonstrated that PGAM2 locates both in cytoplasm and nuclei, and takes part in the glycometabolism process of cytoplasm and nuclei(PMID: 18499067). Defects in PGAM2 are the cause of glycogen storage disease type 10 (GSD10). This antibody may also recognize PGAM1 and PGAM4 due to the high homology.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 15550-1-AP (same clone as 15550-1-PBS)

WB analysis of HeLa using 15550-1-AP

WB analysis of mouse skeletal muscle using 15550-1-AP (same clone as 15550-1-PBS)

WB analysis of mouse skeletal muscle using 15550-1-AP (same clone as 15550-1-PBS)

WB analysis of mouse heart using 15550-1-AP (same clone as 15550-1-PBS)

IHC staining of human breast cancer using 15550-1-AP (same clone as 15550-1-PBS)

IHC staining of human breast cancer using 15550-1-AP (same clone as 15550-1-PBS)

IHC staining of mouse heart using 15550-1-AP (same clone as 15550-1-PBS)

IHC staining of mouse heart using 15550-1-AP (same clone as 15550-1-PBS)

IHC staining of mouse brain using 15550-1-AP (same clone as 15550-1-PBS)