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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated PEDF Monoclonal antibody

PEDF Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2C5C8

Cat no : CL488-66554

Synonyms

EPC 1, PEDF, PIG35, Serpin F1, SERPINF1

Validation Data Gallery

Applications:
  • WB analysis using CL488-66554

    WB analysis using CL488-66554

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66554 (PEDF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inpig plasma, human plasma
Planning an IHC experiment? We recommend our IHCeasy SERPINF1/PEDF Ready-To-Use IHC Kit. SERPINF1/PEDF primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66554-1-Ig

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Product Information

CL488-66554 targets PEDF in WB applications and shows reactivity with Human, pig samples.

Tested Reactivity Human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PEDF fusion protein Ag26660 相同性解析による交差性が予測される生物種
Full Name serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
Calculated molecular weight 418 aa, 46 kDa
Observed molecular weight 46-50 kDa
GenBank accession numberBC013984
Gene symbol SERPINF1
Gene ID (NCBI) 5176
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PEDF also known as serpin F1 (SERPINF1), is a multifunctional secreted protein that has anti-angiogenic, anti-tumorigenic, and neurotrophic functionsis. It is an approximately 50-kDa secreted protein that is widely expressed, including by osteoblasts and osteoclasts. PEDF is also one of the most abundant secretory products of adipocytes, and circulating concentrations of PEDF correlate positively with body fat mass and INS resistance. PEDF is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells. Mutations in this gene were found in individuals with osteogenesis imperfecta, type VI.

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 PEDF antibody CL488-66554Download protocol
Standard Protocols
Click here to view our Standard Protocols