Validation Data Gallery
Tested Applications
| Positive WB detected in | C2C12 cells, Caco-2 cells, HEK-293T cells, Saos-2 cells, rat lung tissue, human heart tissue |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85027-5-RR targets PDLIM1/CLP36 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | PDLIM1/CLP36 fusion protein Ag2274 相同性解析による交差性が予測される生物種 |
| Full Name | PDZ and LIM domain 1 |
| Calculated molecular weight | 329 aa, 36 kDa |
| Observed molecular weight | 36 kDa |
| GenBank accession number | BC000915 |
| Gene Symbol | PDLIM1/CLP36 |
| Gene ID (NCBI) | 9124 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O00151 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PDLIM1, also named as CLIM1, CLP-36, CLP36, hCLIM1 and Elfin, is a member of the actinin-associated LIM protein (ALP)/enigma protein family, plays a role in neurite outgrowth in the peripheral nervous system. It is a cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton. PDLIM1 and PALLD formed a complex in the dorsal root ganglion (DRG) and PDLIM1 and PALLD were colocalized in the neurites and cell bodies of primary DRG neurons. PDLIM1 may interact with PALLD to influence neurite outgrowth during sciatic nerve regeneration. (PMID: 20381583)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PDLIM1/CLP36 antibody 85027-5-RR | Download protocol |
| IF protocol for PDLIM1/CLP36 antibody 85027-5-RR | Download protocol |
| FC protocol for PDLIM1/CLP36 antibody 85027-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |