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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PDLIM1/CLP36 Recombinant monoclonal antibody (85027-5-RR)

    WB analysis using 85027-5-RR (same clone as 85027-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85027-5-RR (PDLIM1,CLP36 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85027-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using PDLIM1/CLP36 Recombinant monoclonal antibody (85027-5-RR)

    IHC staining of human stomach cancer using 85027-5-RR (same clone as 85027-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 85027-5-RR (PDLIM1/CLP36 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85027-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PDLIM1/CLP36 Recombinant monoclonal antibody (85027-5-RR)

    IF Staining of HeLa using 85027-5-RR (same clone as 85027-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PDLIM1/CLP36 antibody (85027-5-RR, Clone: 242585C7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85027-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using PDLIM1/CLP36 Recombinant monoclonal antibody (85027-5-RR)

    FC experiment of HepG2 using 85027-5-RR (same clone as 85027-5-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PDLIM1/CLP36 Recombinant antibody (85027-5-RR, Clone:242585C7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 85027-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85027-5-RR

    Affinity and Kinetic Characterization of 85027-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85027-5-RR against Human PDLIM1/CLP36 were performed. The affinity constant is 6.23 nM.

PDLIM1/CLP36 Recombinant monoclonal antibody, PBS Only

PDLIM1/CLP36 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 85027-5-PBS
Clone No.242585C7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

PDLIM1, 242585C7, CLIM1, CLP36, CLP-36

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85027-5-PBS targets PDLIM1/CLP36 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag2274

Product name: Recombinant human PDLIM1,CLP36 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-329 aa of BC000915

Sequence: MTTQQIDLQGPGPWGFRLVGGKDFEQPLAISRVTPGSKAALANLCIGDVITAIDGENTSNMTHLEAQNRIKGCTDNLTLTVARSEHKVWSPLVTEEGKRHPYKMNLASEPQEVLHIGSAHNRSAMPFTASPASSTTARVITNQYNNPAGLYSSENISNFNNALESKTAASGVEANSRPLDHAQPPSSLVIDKESEVYKMLQEKQELNEPPKQSTSFLVLQEILESEEKGDPNKPSGFRSVKAPVTKVAASIGNAQKLPMCDKCGTGIVGVFVKLRDRHRHPECYVCTDCGTNLKQKGHFFVEDQIYCEKHARERVTPPEGYEVVTVFPK

相同性解析による交差性が予測される生物種
Full Name PDZ and LIM domain 1
Calculated molecular weight 329 aa, 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC000915
Gene Symbol PDLIM1/CLP36
Gene ID (NCBI) 9124
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO00151
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PDLIM1, also named as CLIM1, CLP-36, CLP36, hCLIM1 and Elfin, is a member of the actinin-associated LIM protein (ALP)/enigma protein family, plays a role in neurite outgrowth in the peripheral nervous system. It is a cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton. PDLIM1 and PALLD formed a complex in the dorsal root ganglion (DRG) and PDLIM1 and PALLD were colocalized in the neurites and cell bodies of primary DRG neurons. PDLIM1 may interact with PALLD to influence neurite outgrowth during sciatic nerve regeneration. (PMID: 20381583)

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