• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated PDIA3 Monoclonal antibody

PDIA3 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3G4G7

Cat no : CL594-66423

Synonyms

58 kDa microsomal protein, Disulfide isomerase ER 60, ER protein 57, ER protein 60, ER60, ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PDIA3, PI PLC, Protein disulfide isomerase A3

Validation Data Gallery

Applications:
  • IF Staining of U2OS using CL594-66423

    IF Staining of U2OS using CL594-66423

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite®594 PDIA3 antibody (CL594-66423, Clone: 3G4G7 ) at dilution of 1:200.

Tested Applications

Positive IF detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy PDIA3 Ready-To-Use IHC Kit. PDIA3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66423-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-66423 targets PDIA3 in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PDIA3 fusion protein Ag8741 相同性解析による交差性が予測される生物種
Full Name protein disulfide isomerase family A, member 3
Calculated molecular weight 505 aa, 57 kDa
Observed molecular weight 57 kDa, 60 kDa
GenBank accession numberBC014433
Gene symbol PDIA3
Gene ID (NCBI) 2923
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDIA3, also named as P58, ER60, ERp57, ERp60, ERp61, GRP57, GRP58 and PI-PLC, is a member of the PDI family, participates in the oxidation, reduction, and isomerization of disulfide bonds for correct folding of secretory proteins before modification and transport in the endoplasmic reticulum. It is associated with apoptosis or inhibition of cancer cell growth. PDIA3 was once thought to be a phospholipase; however, it has been demonstrated that this protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and PDIA3 mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.

Protocols

Product Specific Protocols
IF protocol for CL594 PDIA3 antibody CL594-66423Download protocol
Standard Protocols
Click here to view our Standard Protocols