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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PDHB Monoclonal antibody (68238-1-Ig)

    WB analysis using 68238-1-Ig (same clone as 68238-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68238-1-Ig (PDHB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68238-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using PDHB Monoclonal antibody (68238-1-Ig)

    IHC staining of mouse heart using 68238-1-Ig (same clone as 68238-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68238-1-Ig (PDHB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68238-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using PDHB Monoclonal antibody (68238-1-Ig)

    IHC staining of mouse heart using 68238-1-Ig (same clone as 68238-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68238-1-Ig (PDHB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68238-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat stomach tissue using PDHB Monoclonal antibody (68238-1-Ig)

    IHC staining of rat stomach using 68238-1-Ig (same clone as 68238-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 68238-1-Ig (PDHB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68238-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat stomach tissue using PDHB Monoclonal antibody (68238-1-Ig)

    IHC staining of rat stomach using 68238-1-Ig (same clone as 68238-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 68238-1-Ig (PDHB antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68238-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using PDHB Monoclonal antibody (68238-1-Ig)

    IHC staining of mouse kidney using 68238-1-Ig (same clone as 68238-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68238-1-Ig (PDHB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68238-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using PDHB Monoclonal antibody (68238-1-Ig)

    IHC staining of mouse kidney using 68238-1-Ig (same clone as 68238-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68238-1-Ig (PDHB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68238-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using PDHB Monoclonal antibody (68238-1-Ig)

    IHC staining of mouse skeletal muscle using 68238-1-Ig (same clone as 68238-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68238-1-Ig (PDHB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68238-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using PDHB Monoclonal antibody (68238-1-Ig)

    IHC staining of mouse skeletal muscle using 68238-1-Ig (same clone as 68238-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68238-1-Ig (PDHB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68238-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat skeletal muscle tissue using PDHB Monoclonal antibody (68238-1-Ig)

    IHC staining of rat skeletal muscle using 68238-1-Ig (same clone as 68238-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 68238-1-Ig (PDHB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68238-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat skeletal muscle tissue using PDHB Monoclonal antibody (68238-1-Ig)

    IHC staining of rat skeletal muscle using 68238-1-Ig (same clone as 68238-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 68238-1-Ig (PDHB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68238-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PDHB Monoclonal antibody (68238-1-Ig)

    IF Staining of HeLa using 68238-1-Ig (same clone as 68238-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PDHB antibody (68238-1-Ig, Clone: 2A7C10 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68238-1-PBS in a different storage buffer formulation.

PDHB Monoclonal antibody, PBS Only

PDHB Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68238-1-PBS
Clone No.2A7C10

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

2A7C10, EC:1.2.4.1, PDHE1B, PDHE1-B, PHE1B

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68238-1-PBS targets PDHB in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag6857

Product name: Recombinant human PDHB protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-359 aa of BC000439

Sequence: MAAVSGLVRRPLREVSGLLKRRFHWTAPAALQVTVRDAINQGMDEELERDEKVFLLGEEVAQYDGAYKVSRGLWKKYGDKRIIDTPISEMGFAGIAVGAAMAGLRPICEFMTFNFSMQAIDQVINSAAKTYYMSGGLQPVPIVFRGPNGASAGVAAQHSQCFAAWYGHCPGLKVVSPWNSEDAKGLIKSAIRDNNPVVVLENELMYGVPFEFPPEAQSKDFLIPIGKAKIERQGTHITVVSHSRPVGHCLEAAAVLSKEGVECEVINMRTIRPMDMETIEASVMKTNHLVTVEGGWPQFGVGAEICARIMEGPAFNFLDAPAVRVTGADVPMPYAKILEDNSIPQVKDIIFAIKKTLNI

相同性解析による交差性が予測される生物種
Full Name pyruvate dehydrogenase (lipoamide) beta
Calculated molecular weight 39 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC000439
Gene Symbol PDHB
Gene ID (NCBI) 5162
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP11177
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PDHB (Pyruvate dehydrogenase E1 component subunit beta, mitochondrial) is also named PHE1B. The enzyme, which is found in mitochondria, is one of the component enzymes of the pyruvate dehydrogenase multienzyme complex (PDH). It catalyzes the first reaction of an oxidative decarboxylation sequence converting pyruvate to acetyl-CoA and CO(2)(PMID:3422424). Defects in PDHB are the cause of pyruvate dehydrogenase E1-beta deficiency (PDHBD)(PMID:15138885).

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