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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PDGFR beta/CD140b Recombinant antibody (82943-1-RR)

    WB analysis using 82943-1-RR (same clone as 82943-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82943-1-RR (PDGFRB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82943-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PDGFR beta/CD140b Recombinant antibody (82943-1-RR)

    WB analysis using 82943-1-RR (same clone as 82943-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82943-1-RR (PDGFRB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82943-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PDGFR beta/CD140b Recombinant antibody (82943-1-RR)

    IHC staining of human placenta using 82943-1-RR (same clone as 82943-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82943-1-RR (PDGFR beta antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82943-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PDGFR beta/CD140b Recombinant antibody (82943-1-RR)

    IHC staining of human placenta using 82943-1-RR (same clone as 82943-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82943-1-RR (PDGFR beta antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82943-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82943-1-RR

    Affinity and Kinetic Characterization of 82943-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82943-1-RR against Human PDGFRB were performed. The affinity constant is 0.668 nM.

PDGFR beta/CD140b Recombinant monoclonal antibody, PBS Only

PDGFR beta/CD140b Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 82943-1-PBS
Clone No.230214D11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

PDGFRB, EC:2.7.10.1, CD140B, CD140 antigen-like family member B, Beta-type platelet-derived growth factor receptor

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82943-1-PBS targets PDGFR beta/CD140b in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Recombinant protein

相同性解析による交差性が予測される生物種
Full Name platelet-derived growth factor receptor, beta polypeptide
Calculated molecular weight 1106 aa, 124 kDa
Observed molecular weight180 kDa
GenBank accession numberBC032224
Gene Symbol PDGFR beta
Gene ID (NCBI) 5159
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP09619
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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