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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PDGFR beta Recombinant antibody (82943-1-RR)

    WB analysis using 82943-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82943-1-RR (PDGFRB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PDGFR beta Recombinant antibody (82943-1-RR)

    WB analysis using 82943-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82943-1-RR (PDGFRB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PDGFR beta Recombinant antibody (82943-1-RR)

    IHC staining of human placenta using 82943-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82943-1-RR (PDGFR beta antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using PDGFR beta Recombinant antibody (82943-1-RR)

    IHC staining of human placenta using 82943-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82943-1-RR (PDGFR beta antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82943-1-RR

    Affinity and Kinetic Characterization of 82943-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82943-1-RR against Human PDGFRB were performed. The affinity constant is 0.668 nM.

PDGFR beta/CD140b Recombinant monoclonal antibody

PDGFR beta/CD140b Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 82943-1-RR
Clone No.230214D11
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Synonyms

PDGFRB, EC:2.7.10.1, CD140B, CD140 antigen-like family member B, Beta-type platelet-derived growth factor receptor

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMG-63 cells, HeLa cells, COLO 320 cells, U-87 MG cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

82943-1-RR targets PDGFR beta/CD140b in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Recombinant protein

相同性解析による交差性が予測される生物種
Full Name platelet-derived growth factor receptor, beta polypeptide
Calculated molecular weight 1106 aa, 124 kDa
Observed molecular weight180 kDa
GenBank accession numberBC032224
Gene Symbol PDGFR beta
Gene ID (NCBI) 5159
RRIDAB_3670686
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP09619
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for PDGFR beta/CD140b antibody 82943-1-RRDownload protocol
WB protocol for PDGFR beta/CD140b antibody 82943-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Acta Diabetol

The UBR5 protein facilitates mesangial cell hypertrophy and glycolysis induced by high glucose by increasing the phosphorylation levels of AKT

Authors - Lin Liao
View Article
humanWB

Stem Cells Int

Human Umbilical Cord-Mesenchymal Stem Cells Combined With Low Dosage Nintedanib Rather Than Using Alone Mitigates Pulmonary Fibrosis in Mice

Authors - Huijun Qiu
View Article
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