Validation Data Gallery
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Tested Applications
| Positive FC detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL647-98256 targets PDGFR alpha/CD140a in FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | PDGFR alpha/CD140a fusion protein Eg1625 相同性解析による交差性が予測される生物種 |
| Full Name | platelet derived growth factor receptor, alpha polypeptide |
| Calculated molecular weight | 123kDa |
| GenBank accession number | NM_001083316.2 |
| Gene Symbol | Pdgfra |
| Gene ID (NCBI) | 18595 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P26618-1 |
| Storage Buffer | PBS with 0.09% sodium azide{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
Platelet-derived growth factor receptor alpha (PDGFR alpha, also known as CD140a) is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. PDGFRA is implicated in the regulation of embryonic development, tumorigenesis, cell proliferation, survival, and chemotaxis. Mutations in the PDGFRA gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 PDGFR alpha/CD140a antibody CL647-98256 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |