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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PDE8B Polyclonal antibody (30708-1-AP)

    WB analysis using 30708-1-AP (same clone as 30708-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30708-1-AP (PDE8B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30708-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PDE8B Polyclonal antibody (30708-1-AP)

    IHC staining of mouse testis using 30708-1-AP (same clone as 30708-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30708-1-AP (PDE8B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30708-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PDE8B Polyclonal antibody (30708-1-AP)

    IHC staining of mouse testis using 30708-1-AP (same clone as 30708-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30708-1-AP (PDE8B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30708-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using PDE8B Polyclonal antibody (30708-1-AP)

    IF Staining of mouse testis using 30708-1-AP (same clone as 30708-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PDE8B antibody (30708-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30708-1-PBS in a different storage buffer formulation.

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PDE8B Polyclonal antibody, PBS Only

PDE8B Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 30708-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

PPNAD3, phosphodiesterase 8B, HsPDE8B

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30708-1-PBS targets PDE8B in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDE8B fusion protein Ag33477 相同性解析による交差性が予測される生物種
Full Name phosphodiesterase 8B
Calculated molecular weight99kd
Observed molecular weight68-98 kDa
GenBank accession numberNM_003719
Gene Symbol PDE8B
Gene ID (NCBI) 8622
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95263
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Human phosphodiesterase (PDE) type 8B (PDE8B) is located at 5q14.1 and is known as the PDE with the highest affinity to cAMP.