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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PDE8B Polyclonal antibody

PDE8B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30708-1-AP

Synonyms

FLJ11212, HsPDE8B, PDE8B, phosphodiesterase 8B, PPNAD3

Validation Data Gallery

Applications:
  • WB analysis using 30708-1-AP

    WB analysis using 30708-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30708-1-AP (PDE8B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 30708-1-AP

    IHC staining of mouse testis using 30708-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30708-1-AP (PDE8B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 30708-1-AP

    IHC staining of mouse testis using 30708-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30708-1-AP (PDE8B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse brain tissue, human placenta tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

30708-1-AP targets PDE8B in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDE8B fusion protein Ag33477 相同性解析による交差性が予測される生物種
Full Name phosphodiesterase 8B
Calculated molecular weight99kd
Observed molecular weight68-98 kDa
GenBank accession numberNM_003719
Gene symbol PDE8B
Gene ID (NCBI) 8622
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human phosphodiesterase (PDE) type 8B (PDE8B) is located at 5q14.1 and is known as the PDE with the highest affinity to cAMP.

Protocols

Product Specific Protocols
WB protocol for PDE8B antibody 30708-1-APDownload protocol
IHC protocol for PDE8B antibody 30708-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols