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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using PDE5A Recombinant antibody (85030-1-RR)

    WB analysis of mouse lung using 85030-1-RR

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 85030-1-RR (PDE5A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat lung tissue using PDE5A Recombinant antibody (85030-1-RR)

    WB analysis of rat lung using 85030-1-RR

    rat lung tissue were subjected to SDS PAGE followed by western blot with 85030-1-RR (PDE5A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of SKOV-3 cells using PDE5A Recombinant antibody (85030-1-RR)

    IF Staining of SKOV-3 using 85030-1-RR

    Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using PDE5A antibody (85030-1-RR, Clone: 242597B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 85030-1-RR

    Affinity and Kinetic Characterization of 85030-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85030-1-RR against Human PDE5A were performed. The affinity constant is 1.07 nM.

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PDE5A Recombinant antibody

PDE5A Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85030-1-RR
Clone No.242597B10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

EC:3.1.4.35, CN5A, cGMP-specific 3',5'-cyclic phosphodiesterase, cGMP-binding cGMP-specific phosphodiesterase, CGB-PDE

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse lung tissue, rat lung tissue
Positive IF/ICC detected inSKOV-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85030-1-RR targets PDE5A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PDE5A fusion protein Ag18779 相同性解析による交差性が予測される生物種
Full Name phosphodiesterase 5A, cGMP-specific
Calculated molecular weight 875 aa, 100 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC126233
Gene Symbol PDE5A
Gene ID (NCBI) 8654
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO76074
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDE5A (cGMP-binding cGMP-specific phosphodiesterase) plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP. Through alternative splicing, the PDE5A gene produces three proteins (PDE5A1, PDE5A2, and PDE5A3) that differ in their N termini and range in mass from 89 to 105 kDa. (PMID: 25704994; 21215707). A second 70 kDa band was consistently observed in heart tissue that either reflected a splice variant or proteolytic fragment of PDE5A. (PMID: 15576651).

Protocols

Product Specific Protocols
WB protocol for PDE5A antibody 85030-1-RRDownload protocol
IF protocol for PDE5A antibody 85030-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols