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Validation Data Gallery

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  • Western Blot (WB) analysis of rat lung tissue using PDE5A Recombinant monoclonal antibody (85030-1-RR)

    WB analysis of rat lung using 85030-1-RR (same clone as 85030-1-PBS)

    rat lung tissue were subjected to SDS PAGE followed by western blot with 85030-1-RR (PDE5A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85030-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using PDE5A Recombinant monoclonal antibody (85030-1-RR)

    WB analysis of mouse lung using 85030-1-RR (same clone as 85030-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 85030-1-RR (PDE5A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85030-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP01762-3

    Sandwich ELISA sample test of MP01762-3

    The mean PDE5A concentration was determined to be 34.07 ng/mL in HeLa cell extract based on a 1.5 mg/mL extract load and 35.64 ng/mL in MCF-7 cell extract based on a 1.2 mg/mL extract load.

  • Sandwich ELISA standard curve of MP01762-3

    Sandwich ELISA standard curve of MP01762-3

    Sandwich ELISA standard curve of MP01762-3, Human PDE5A Recombinant Matched Antibody Pair - PBS only. 85030-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag18779. 85030-1-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using PDE5A Recombinant monoclonal antibody (85030-1-RR)

    IHC staining of human ovary cancer using 85030-1-RR (same clone as 85030-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 85030-1-RR (PDE5A antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85030-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SKOV-3 cells using PDE5A Recombinant monoclonal antibody (85030-1-RR)

    IF Staining of SKOV-3 using 85030-1-RR (same clone as 85030-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using PDE5A antibody (85030-1-RR, Clone: 242597B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85030-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293 cells using PDE5A Recombinant monoclonal antibody (85030-1-RR)

    FC experiment of HEK-293 using 85030-1-RR (same clone as 85030-1-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug PDE5A Recombinant antibody (85030-1-RR, Clone:242597B10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 85030-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85030-1-RR

    Affinity and Kinetic Characterization of 85030-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85030-1-RR against Human PDE5A were performed. The affinity constant is 1.07 nM.

PDE5A Recombinant monoclonal antibody, PBS Only (Detector)

PDE5A Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA
Cat No. 85030-1-PBS
Clone No.242597B10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA

Synonyms

242597B10, CGB-PDE, cGMP-binding cGMP-specific phosphodiesterase, cGMP-specific 3',5'-cyclic phosphodiesterase, CN5A

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85030-1-PBS targets PDE5A as part of a matched antibody pair:

MP01762-3: 85030-4-PBS capture and 85030-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag18779

Product name: Recombinant human PDE5A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 321-482 aa of BC126233

Sequence: ETSLLENKRNQVLLDLASLIFEEQQSLEVILKKIAATIISFMQVQKCTIFIVDEDCSDSFSSVFHMECEELEKSSDTLTREHDANKINYMYAQYVKNTMEPLNIPDVSKDKRFPWTTENTGNVNQQCIRSLLCTPIKNGKKNKVIGVCQLVNKMEENTGKVK

相同性解析による交差性が予測される生物種
Full Name phosphodiesterase 5A, cGMP-specific
Calculated molecular weight 875 aa, 100 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC126233
Gene Symbol PDE5A
Gene ID (NCBI) 8654
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO76074
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PDE5A (cGMP-binding cGMP-specific phosphodiesterase) plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP. Through alternative splicing, the PDE5A gene produces three proteins (PDE5A1, PDE5A2, and PDE5A3) that differ in their N termini and range in mass from 89 to 105 kDa. (PMID: 25704994; 21215707). A second 70 kDa band was consistently observed in heart tissue that either reflected a splice variant or proteolytic fragment of PDE5A. (PMID: 15576651).