Validation Data Gallery
Tested Applications
| Positive FC detected in | anti-CD3/CD28 treated mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.1 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
PY7-FcA98166-4 targets PD-L1/CD274 in FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
DNA 相同性解析による交差性が予測される生物種 |
| Full Name | CD274 antigen |
| Calculated molecular weight | 33 kDa |
| GenBank accession number | NM_021893.3 |
| Gene Symbol | PD-L1 |
| Gene ID (NCBI) | 60533 |
| Conjugate | PE-Cyanine7 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 496 nm, 565 nm / 778 nm |
| 激发激光 | Blue Laser (488 nm), Green Laser (532 nm), Yellow-Green Laser (561 nm) |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9EP73 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1) is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cells, and plays an important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL-10 and IFN gamma, in an IL-2-dependent and a PD-1-independent manner.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PE-Cyanine7 PD-L1/CD274 antibody PY7-FcA98166-4 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |