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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PCNXL2 Polyclonal antibody (20417-1-AP)

    WB analysis of HeLa using 20417-1-AP (same clone as 20417-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 20417-1-AP (PCNXL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20417-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using PCNXL2 Polyclonal antibody (20417-1-AP)

    WB analysis of MCF-7 using 20417-1-AP (same clone as 20417-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 20417-1-AP (PCNXL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20417-1-PBS in a different storage buffer formulation.

PCNXL2 Polyclonal antibody, PBS Only

PCNXL2 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 20417-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20417-1-PBS targets PCNXL2 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14161

Product name: Recombinant human PCNXL2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1108-1201 aa of BC008300

Sequence: LSFAVSASTVFLSLRPFLSIVLFALAGAVGFVTHYVLPQLRKHHPWMWISHPILKNKEYHQREVRDVAHLMWFERLYVWLQCFEKYILYPALIL

相同性解析による交差性が予測される生物種
Full Name pecanex-like 2 (Drosophila)
Calculated molecular weight 2137 aa, 237 kDa
Observed molecular weight 237-260 kDa
GenBank accession numberBC008300
Gene Symbol PCNXL2
Gene ID (NCBI) 80003
RRIDAB_10792807
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDA6NKB5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PCNXL2, also named as KIAA0435, belongs to the pecanex family. It may play a role in tumorigenesis of colorectal carcinomas with high microsatellite instability (MSI-H). PCNXL2 is characterized by high mutational frequencies and biallelic mutations in MSI-H colorectal tumors, and is thus likely to be a target gene in these tumors. PCNXL2 is a glycosylation protein. The MW migrates to 260kd.

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