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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PCNXL2 Polyclonal antibody (20417-1-AP)

    WB analysis of HeLa using 20417-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20417-1-AP (PCNXL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using PCNXL2 Polyclonal antibody (20417-1-AP)

    WB analysis of MCF-7 using 20417-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 20417-1-AP (PCNXL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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PCNXL2 Polyclonal antibody

PCNXL2 Polyclonal Antibody for WB, ELISA
Cat No. 20417-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

KIAA0435, PCNXL2, pecanex like 2 (Drosophila), Pecanex like protein 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20417-1-AP targets PCNXL2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCNXL2 fusion protein Ag14161 相同性解析による交差性が予測される生物種
Full Name pecanex-like 2 (Drosophila)
Calculated molecular weight 2137 aa, 237 kDa
Observed molecular weight 237-260 kDa
GenBank accession numberBC008300
Gene Symbol PCNXL2
Gene ID (NCBI) 80003
RRIDAB_10792807
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA6NKB5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PCNXL2, also named as KIAA0435, belongs to the pecanex family. It may play a role in tumorigenesis of colorectal carcinomas with high microsatellite instability (MSI-H). PCNXL2 is characterized by high mutational frequencies and biallelic mutations in MSI-H colorectal tumors, and is thus likely to be a target gene in these tumors. PCNXL2 is a glycosylation protein. The MW migrates to 260kd.

Protocols

Product Specific Protocols
WB protocol for PCNXL2 antibody 20417-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols