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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCMTD1 Polyclonal antibody (24425-1-AP)

    WB analysis using 24425-1-AP (same clone as 24425-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24425-1-AP (PCMTD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24425-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PCMTD1 Polyclonal antibody (24425-1-AP)

    WB analysis using 24425-1-AP (same clone as 24425-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24425-1-AP (PCMTD1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24425-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PCMTD1 Polyclonal antibody (24425-1-AP)

    WB analysis using 24425-1-AP (same clone as 24425-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24425-1-AP (PCMTD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24425-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PCMTD1 Polyclonal antibody (24425-1-AP)

    IF Staining of HepG2 using 24425-1-AP (same clone as 24425-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PCMTD1 antibody (24425-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24425-1-PBS in a different storage buffer formulation.

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PCMTD1 Polyclonal antibody, PBS Only

PCMTD1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 24425-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24425-1-PBS targets PCMTD1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCMTD1 fusion protein Ag19634 相同性解析による交差性が予測される生物種
Full Name protein-L-isoaspartate (D-aspartate) O-methyltransferase domain containing 1
Calculated molecular weight 357 aa, 41 kDa
Observed molecular weight41 kDa, 27 kDa
GenBank accession numberBC032670
Gene Symbol PCMTD1
Gene ID (NCBI) 115294
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96MG8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Protein-l-isoaspartate O-methyltransferase domain-containing protein 1 (PCMTD1) is a putative E3 ubiquitin ligase substrate adaptor protein, which has a C-terminal domain containing SOCS-box ubiquitin ligase recruitment motifs. PCMTD1 protein may provide an alternate maintenance pathway for modified proteins in mammalian cells (PMID: 35486881). This antibody can detect two isoforms of PCMTD1, 27 kDa and 41 kDa respectively (PMID: 37953789, 35486881).