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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PCMTD1 Polyclonal antibody

PCMTD1 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 24425-1-AP

Synonyms

PCMTD1

Validation Data Gallery

Applications:
  • WB analysis using 24425-1-AP

    WB analysis using 24425-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24425-1-AP (PCMTD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 24425-1-AP

    WB analysis using 24425-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24425-1-AP (PCMTD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 24425-1-AP

    WB analysis using 24425-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24425-1-AP (PCMTD1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 24425-1-AP

    IF Staining of HepG2 using 24425-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PCMTD1 antibody (24425-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inrat liver tissue, human placenta tissue, hTERT-RPE1 cells, mouse eye tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

24425-1-AP targets PCMTD1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCMTD1 fusion protein Ag19634 相同性解析による交差性が予測される生物種
Full Name protein-L-isoaspartate (D-aspartate) O-methyltransferase domain containing 1
Calculated molecular weight 357 aa, 41 kDa
Observed molecular weight41 kDa, 27 kDa
GenBank accession numberBC032670
Gene symbol PCMTD1
Gene ID (NCBI) 115294
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein-l-isoaspartate O-methyltransferase domain-containing protein 1 (PCMTD1) is a putative E3 ubiquitin ligase substrate adaptor protein, which has a C-terminal domain containing SOCS-box ubiquitin ligase recruitment motifs. PCMTD1 protein may provide an alternate maintenance pathway for modified proteins in mammalian cells (PMID: 35486881). This antibody can detect two isoforms of PCMTD1, 27 kDa and 41 kDa respectively (PMID: 37953789, 35486881).

Protocols

Product Specific Protocols
WB protocol for PCMTD1 antibody 24425-1-APDownload protocol
IF protocol for PCMTD1 antibody 24425-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols