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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCMT1 Monoclonal antibody (60172-1-Ig)

    WB analysis using 60172-1-Ig (same clone as 60172-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60172-1-Ig (PCMT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60172-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using PCMT1 Monoclonal antibody (60172-1-Ig)

    WB analysis of pig brain using 60172-1-Ig (same clone as 60172-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 60172-1-Ig (PCMT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60172-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using PCMT1 Monoclonal antibody (60172-1-Ig)

    WB analysis of Raji using 60172-1-Ig (same clone as 60172-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 60172-1-Ig (PCMT1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60172-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PCMT1 Monoclonal antibody (60172-1-Ig)

    IF Staining of HeLa using 60172-1-Ig (same clone as 60172-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PCMT1 antibody (60172-1-Ig, Clone: 3C8G8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60172-1-PBS in a different storage buffer formulation.

PCMT1 Monoclonal antibody, PBS Only

PCMT1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 60172-1-PBS
Clone No.3C8G8

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

3C8G8

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60172-1-PBS targets PCMT1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag6277

Product name: Recombinant human PCMT1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-227 aa of BC008748

Sequence: MAWKSGGASHSELIHNLRKNGIIKTDKVFEVMLATDRSHYAKCNPYMDSPQSIGFQATISAPHMHAYALELLFDQLHEGAKALDVGSGSGILTACFARMVGCTGKVIGIDHIKELVDDSINNVRKDDPTLLSSGRVQLVVGDGRMGYAEEAPYDAIHVGAAAPVVPQALIDQLKPGGRLILPVGPAGGNQMLEQYDKLQDGSIKMKPLMGVIYVPLTDKEKQWSRWK

相同性解析による交差性が予測される生物種
Full Name protein-L-isoaspartate (D-aspartate) O-methyltransferase
Calculated molecular weight 25 kDa
Observed molecular weight 25-27 kDa
GenBank accession numberBC008748
Gene Symbol PCMT1
Gene ID (NCBI) 5110
RRIDAB_10695773
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP22061
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The PCMT1 gene encodes the protein repair enzyme protein-L-isoaspartate (D-aspartate) O-methyltransferase, which is known to protect certain neural cells against Bax-induced apoptosis.