Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, Raji cells, pig brain tissue, HEK-293 cells, HepG2 cells, K-562 cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60172-1-Ig targets PCMT1 in WB, IF/ICC, ELISA applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6277 Product name: Recombinant human PCMT1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-227 aa of BC008748 Sequence: MAWKSGGASHSELIHNLRKNGIIKTDKVFEVMLATDRSHYAKCNPYMDSPQSIGFQATISAPHMHAYALELLFDQLHEGAKALDVGSGSGILTACFARMVGCTGKVIGIDHIKELVDDSINNVRKDDPTLLSSGRVQLVVGDGRMGYAEEAPYDAIHVGAAAPVVPQALIDQLKPGGRLILPVGPAGGNQMLEQYDKLQDGSIKMKPLMGVIYVPLTDKEKQWSRWK 相同性解析による交差性が予測される生物種 |
| Full Name | protein-L-isoaspartate (D-aspartate) O-methyltransferase |
| Calculated molecular weight | 25 kDa |
| Observed molecular weight | 25-27 kDa |
| GenBank accession number | BC008748 |
| Gene Symbol | PCMT1 |
| Gene ID (NCBI) | 5110 |
| RRID | AB_10695773 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P22061 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The PCMT1 gene encodes the protein repair enzyme protein-L-isoaspartate (D-aspartate) O-methyltransferase, which is known to protect certain neural cells against Bax-induced apoptosis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PCMT1 antibody 60172-1-Ig | Download protocol |
| WB protocol for PCMT1 antibody 60172-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
