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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCIF1 Polyclonal antibody (16082-1-AP)

    WB analysis using 16082-1-AP (same clone as 16082-1-PBS)

    Ramos cells were subjected to SDS PAGE followed by western blot with 16082-1-AP (PCIF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16082-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using PCIF1 Polyclonal antibody (16082-1-AP)

    WB analysis of HepG2 using 16082-1-AP (same clone as 16082-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16082-1-AP (PCIF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16082-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using PCIF1 Polyclonal antibody (16082-1-AP)

    WB analysis of Jurkat using 16082-1-AP (same clone as 16082-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16082-1-AP (PCIF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16082-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293T cells using PCIF1 Polyclonal antibody (16082-1-AP)

    WB analysis of HEK-293T using 16082-1-AP (same clone as 16082-1-PBS)

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 16082-1-AP (PCIF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16082-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PCIF1 Polyclonal antibody (16082-1-AP)

    IHC staining of human tonsillitis using 16082-1-AP (same clone as 16082-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16082-1-AP (PCIF1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16082-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PCIF1 Polyclonal antibody (16082-1-AP)

    IHC staining of human tonsillitis using 16082-1-AP (same clone as 16082-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16082-1-AP (PCIF1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16082-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PCIF1 Polyclonal antibody (16082-1-AP)

    IF Staining of MCF-7 using 16082-1-AP (same clone as 16082-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PCIF1 antibody (16082-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green). This data was developed using the same antibody clone with 16082-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using PCIF1 Polyclonal antibody (16082-1-AP)

    IF Staining of Caco-2 using 16082-1-AP (same clone as 16082-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using PCIF1 antibody (16082-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16082-1-PBS in a different storage buffer formulation.

PCIF1 Polyclonal antibody, PBS Only

PCIF1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 16082-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

C20orf67, CAPAM, Cap-specific adenosine methyltransferase, EC:2.1.1.62, hCAPAM

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16082-1-PBS targets PCIF1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9080

Product name: Recombinant human PCIF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 355-704 aa of BC013365

Sequence: DSVEGICSKIYHISLEYVKRIREKHLAILKENNISEEVEAPEVEPRLVYCYPVRLAVSAPPMPSVEMHMENNVVCIRYKGEMVKVSRNYFSKLWLLYRYSCIDDSAFERFLPRVWCLLRRYQMMFGVGLYEGTGLQGSLPVHVFEALHRLFGVSFECFASPLNCYFRQYCSAFPDTDGYFGSRGPCLDFAPLSGSFEANPPFCEELMDAMVSHFERLLESSPEPLSFIVFIPEWREPPTPALTRMEQSRFKRHQLILPAFEHEYRSGSQHICKKEEMHYKAVHNTAVLFLQNDPGFAKWAPTPERLQELSAAYRQSGRSHSSGSSSSSSSEAKDRDSGREQGPSREPHPT

相同性解析による交差性が予測される生物種
Full Name PDX1 C-terminal inhibiting factor 1
Calculated molecular weight 704 aa, 81 kDa
Observed molecular weight 81 kDa
GenBank accession numberBC013365
Gene Symbol PCIF1
Gene ID (NCBI) 63935
RRIDAB_2159996
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H4Z3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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