Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 16082-1-AP
Ramos cells were subjected to SDS PAGE followed by western blot with 16082-1-AP (PCIF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 16082-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16082-1-AP (PCIF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 16082-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 16082-1-AP (PCIF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293T using 16082-1-AP
HEK-293T cells were subjected to SDS PAGE followed by western blot with 16082-1-AP (PCIF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IHC staining of human tonsillitis using 16082-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16082-1-AP (PCIF1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 16082-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16082-1-AP (PCIF1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 16082-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PCIF1 antibody (16082-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).
IF Staining of Caco-2 using 16082-1-AP
Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using PCIF1 antibody (16082-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

PCIF1 Polyclonal antibody

PCIF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 16082-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

C20orf67, CAPAM, Cap-specific adenosine methyltransferase, EC:2.1.1.62, hCAPAM

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (6)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Ramos cells, HepG2 cells, Jurkat cells, HEK-293T cells
Positive IHC detected in	human tonsillitis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells, Caco-2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 6 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

16082-1-AP targets PCIF1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9080 Product name: Recombinant human PCIF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 355-704 aa of BC013365 Sequence: DSVEGICSKIYHISLEYVKRIREKHLAILKENNISEEVEAPEVEPRLVYCYPVRLAVSAPPMPSVEMHMENNVVCIRYKGEMVKVSRNYFSKLWLLYRYSCIDDSAFERFLPRVWCLLRRYQMMFGVGLYEGTGLQGSLPVHVFEALHRLFGVSFECFASPLNCYFRQYCSAFPDTDGYFGSRGPCLDFAPLSGSFEANPPFCEELMDAMVSHFERLLESSPEPLSFIVFIPEWREPPTPALTRMEQSRFKRHQLILPAFEHEYRSGSQHICKKEEMHYKAVHNTAVLFLQNDPGFAKWAPTPERLQELSAAYRQSGRSHSSGSSSSSSSEAKDRDSGREQGPSREPHPT 相同性解析による交差性が予測される生物種
Full Name	PDX1 C-terminal inhibiting factor 1
Calculated molecular weight	704 aa, 81 kDa
Observed molecular weight	81 kDa
GenBank accession number	BC013365
Gene Symbol	PCIF1
Gene ID (NCBI)	63935
RRID	AB_2159996
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H4Z3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for PCIF1 antibody 16082-1-AP	Download protocol
IHC protocol for PCIF1 antibody 16082-1-AP	Download protocol
WB protocol for PCIF1 antibody 16082-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse,human	WB,IP	Mol Cell RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits Authors - Yuanjun Li View Article
human	WB	Nat Commun HIV reprograms host m 6 Am RNA methylome by viral Vpr protein-mediated degradation of PCIF1 Authors - Qiong Zhang View Article
	WB	J Virol The pseudorabies virus UL13 protein kinase triggers phosphorylation of the RNA demethylase FTO, which is associated with FTO-dependent suppression of interferon-stimulated gene expression Authors - Ruth Verhamme View Article
pig	WB	Cell Rep Alphaherpesvirus US3 protein-mediated inhibition of the m6A mRNA methyltransferase complex. Authors - Robert J J Jansens View Article
mouse,human	WB,IF	EMBO J Role of PCIF1-mediated 5\'-cap N6-methyladeonsine mRNA methylation in colorectal cancer and anti-PD-1 immunotherapy Authors - Lingling Wang View Article
human	WB	Proc Natl Acad Sci U S A PCIF1-mediated deposition of 5'-cap N6,2'-O-dimethyladenosine in ACE2 and TMPRSS2 mRNA regulates susceptibility to SARS-CoV-2 infection Authors - Lingling Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 16082-1-AP

WB analysis of HepG2 using 16082-1-AP

WB analysis of Jurkat using 16082-1-AP

WB analysis of HEK-293T using 16082-1-AP

IHC staining of human tonsillitis using 16082-1-AP

IHC staining of human tonsillitis using 16082-1-AP

IF Staining of MCF-7 using 16082-1-AP

IF Staining of Caco-2 using 16082-1-AP

PCIF1 Polyclonal antibody

PCIF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits

HIV reprograms host m 6 Am RNA methylome by viral Vpr protein-mediated degradation of PCIF1

The pseudorabies virus UL13 protein kinase triggers phosphorylation of the RNA demethylase FTO, which is associated with FTO-dependent suppression of interferon-stimulated gene expression

Alphaherpesvirus US3 protein-mediated inhibition of the m6A mRNA methyltransferase complex.

Role of PCIF1-mediated 5\'-cap N6-methyladeonsine mRNA methylation in colorectal cancer and anti-PD-1 immunotherapy

PCIF1-mediated deposition of 5'-cap N6,2'-O-dimethyladenosine in ACE2 and TMPRSS2 mRNA regulates susceptibility to SARS-CoV-2 infection