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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCARE Recombinant antibody (83364-4-RR)

    WB analysis using 83364-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83364-4-RR (PCARE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using PCARE Recombinant antibody (83364-4-RR)

    IHC staining of mouse eye using 83364-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 83364-4-RR (PCARE antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse eye tissue using PCARE Recombinant antibody (83364-4-RR)

    IHC staining of mouse eye using 83364-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 83364-4-RR (PCARE antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using PCARE Recombinant antibody (83364-4-RR)

    IF Staining of mouse eye using 83364-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PCARE antibody (83364-4-RR, Clone: 240352F6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using PCARE Recombinant antibody (83364-4-RR)

    IF Staining of mouse eye using 83364-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PCARE antibody (83364-4-RR, Clone: 240352F6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83364-4-RR

    Affinity and Kinetic Characterization of 83364-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83364-4-RR against Human PCARE were performed. The affinity constant is 3.06 nM.

PCARE Recombinant monoclonal antibody

PCARE Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 83364-4-RR
Clone No.240352F6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Synonyms

C2orf71, 240352F6

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse retina tissue, mouse eye tissue
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse eye tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83364-4-RR targets PCARE in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag34774

Product name: Recombinant human PCARE protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 10-150 aa of NM_001029883

Sequence: LVNSVAKSGIQFLKKPKAIRPGCQGGSERGSIPLLVKNSTCYDAGEGLAEEQPSPRRNQTTAKGLCQLMGDPASGKRKDMEGLIPGTKTSSSQLNKSQSHMAKDIPFKTQGSHGSQGADFSGDESEESSTQDTSKWKRTAK

相同性解析による交差性が予測される生物種
Full Name chromosome 2 open reading frame 71
Calculated molecular weight140 kDa
Observed molecular weight140 kDa
GenBank accession numberNM_001029883
Gene Symbol PCARE
Gene ID (NCBI) 388939
RRIDAB_3671018
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDA6NGG8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C2orf71/PCARE (photoreceptor cilium actin regulator) is a retina-specific protein consisting of 1289 amino acids with a predicted molecular weight of 140 kDa. PCARE is predicted to be myristoylated and palmitoylated at its N terminus and plays an important role in delivering actin-associated components to the base of the photoreceptor OSs to regulate the initial development of OS disks (PMID: 32312818, 35253837).

Protocols

Product Specific Protocols
IF protocol for PCARE antibody 83364-4-RRDownload protocol
IHC protocol for PCARE antibody 83364-4-RRDownload protocol
WB protocol for PCARE antibody 83364-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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