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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCARE Recombinant antibody (83364-4-RR)

    WB analysis using 83364-4-RR (same clone as 83364-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83364-4-RR (PCARE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83364-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using PCARE Recombinant antibody (83364-4-RR)

    IHC staining of mouse eye using 83364-4-RR (same clone as 83364-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 83364-4-RR (PCARE antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83364-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using PCARE Recombinant antibody (83364-4-RR)

    IHC staining of mouse eye using 83364-4-RR (same clone as 83364-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 83364-4-RR (PCARE antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83364-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using PCARE Recombinant antibody (83364-4-RR)

    IF Staining of mouse eye using 83364-4-RR (same clone as 83364-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PCARE antibody (83364-4-RR, Clone: 240352F6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83364-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using PCARE Recombinant antibody (83364-4-RR)

    IF Staining of mouse eye using 83364-4-RR (same clone as 83364-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PCARE antibody (83364-4-RR, Clone: 240352F6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83364-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83364-4-RR

    Affinity and Kinetic Characterization of 83364-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83364-4-RR against Human PCARE were performed. The affinity constant is 3.06 nM.

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PCARE Recombinant antibody, PBS Only

PCARE Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 83364-4-PBS
Clone No.240352F6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Synonyms

C2orf71, 240352F6

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83364-4-PBS targets PCARE in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PCARE fusion protein Ag34774 相同性解析による交差性が予測される生物種
Full Name chromosome 2 open reading frame 71
Calculated molecular weight140 kDa
Observed molecular weight140 kDa
GenBank accession numberNM_001029883
Gene Symbol PCARE
Gene ID (NCBI) 388939
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDA6NGG8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

C2orf71/PCARE (photoreceptor cilium actin regulator) is a retina-specific protein consisting of 1289 amino acids with a predicted molecular weight of 140 kDa. PCARE is predicted to be myristoylated and palmitoylated at its N terminus and plays an important role in delivering actin-associated components to the base of the photoreceptor OSs to regulate the initial development of OS disks (PMID: 32312818, 35253837).